Regulation and temporal expression patterns of Vibrio cholerae virulence genes during infection
Regulation and temporal expression patterns of Vibrio cholerae virulence genes during infection
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DOI：
10.1016/s0092-8674(00)81551-2
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发表时间：
1999-12-10
期刊：
影响因子：
64.5
通讯作者：
Camilli, A
中科院分区：
文献类型：
--
作者：
Lee, SH;Hava, DL;Camilli, A
使用重组酶报告基因确定感染期间关键霍乱弧菌毒力基因 tcpA 和 ctxA 的时间表达模式。 TcpA 在小肠中的两个时间和空间上可分离的事件中被双相诱导，而 ctxA 仅在 tcpA 表达之后且显着依赖于 tcpA 表达才被单相诱导；然而，在体外生长过程中没有观察到这种依赖性。确定了毒力调节因子 ToxR、TcpP 和 ToxT 对 tcpA 和 ctxA 表达的要求，并发现感染期间与体外生长期间存在显着差异。这些结果说明了在真正的宿主-病原体相互作用的背景下检查毒力基因表达的重要性。
The temporal expression patterns of the critical Vibrio cholerae virulence genes, tcpA and ctxA, were determined during infection using a recombinase reporter. TcpA was induced biphasically in two temporally and spatially separable events in the small intestine, whereas ctxA was induced monophasically only after, and remarkably, dependent upon, tcpA expression; however, this dependence was not observed during in vitro growth. The requirements of the virulence regulators, ToxR, TcpP, and ToxT, for expression of tcpA and ctxA were determined and were found to differ significantly during infection versus during growth in vitro. These results illustrate the importance of examining virulence gene expression in the context of bona fide host-pathogen interactions.