Role of a ubiquitin-like modification in polarized morphogenesis
Role of a ubiquitin-like modification in polarized morphogenesis
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DOI：
10.1126/science.1069989
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发表时间：
2002-03-29
期刊：
影响因子：
56.9
通讯作者：
Finley, D
中科院分区：
文献类型：
--
作者：
Dittmar, GAG;Wilkinson, CRM;Finley, D
I型泛素样蛋白构成蛋白质修饰剂家族。在这里，我们报告的翻译后蛋白质修饰剂从酿酒酵母，集线器1的鉴定。Hub1的过表达导致增强的共轭形成时，其羧基末端残基被删除，这表明成熟的Hub1可能是由蛋白水解加工。Hub1结合的体内靶点包括细胞极性因子Sph1和Hbt1。在hub1Delta突变体中，Hbt1和Sph1的亚细胞定位被破坏，并且在交配突起形成期间的细胞极化是有缺陷的。与这些极化缺陷相一致，hub1Delta突变体在交配中有缺陷。
Type I ubiquitin-like proteins constitute a family of protein modifiers. Here we report the identification of a posttranslational protein modifier from Saccharomyces cerevisiae, Hub1. Overexpression of Hub1 resulted in enhanced conjugate formation when its carboxyl-terminal residue was deleted, suggesting that mature Hub1 may be produced by proteolytic processing. In vivo targets of Hub1 conjugation included cell polarity factors Sph1 and Hbt1. In the hub1Delta mutant, the subcellular localization of both Hbt1 and Sph1 was disrupted, and cell polarization during the formation of mating projections was defective. Consistent with these polarization defects, the hub1Delta mutant was deficient in mating.