Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)
Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)
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DOI：
10.1006/bbrc.1999.0651
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发表时间：
1999-05-19
影响因子：
3.1
通讯作者：
Bruni, CB
中科院分区：
文献类型：
--
作者：
Bucci, C;Chiariello, M;Bruni, CB
Rab蛋白是一种参与哺乳动物细胞内膜运输调节的小GTP酶，为了寻找与Bah相互作用的蛋白，我们利用人脑cDNA文库进行了双杂交筛选。在这里，我们报道了编码185个氨基酸的蛋白质的全长人cDNA克隆的分离。在双杂交实验中，该蛋白与Rab4b、Rab5a和Rab5c蛋白有较强的相互作用，与Rab4a、Rab6、Rab7、Rab17和Rab22有较弱的相互作用，与数据库的比较表明，该克隆代表了先前分离的大鼠Prenylated Rab受体(RPRA1)的人类同源基因。MRNA表达分析显示，约0.8kb的单一丰富的mRNA普遍表达，Western印迹分析显示，预期大小的条带均匀分布在细胞质和细胞膜之间。(C)1999年学术出版社。
Rab proteins are small GTPases involved in the regulation of intracellular membrane traffic in mammalian cells, In order to find Bah-interacting proteins we performed a two-hybrid screening using a human brain cDNA library. Here we report the isolation of a full-length human cDNA clone coding for a protein of 185 amino acids. This protein interacts strongly with the Rab4b, Rab5a, and Rab5c proteins and weakly with Rab4a, Rab6, Rab7, Rab17, and Rab22 in the two-hybrid assay, Comparison with the Data Bank revealed that this clone represents the human homolog of the previously isolated rat Prenylated Rab Acceptor (rPRA1). Analysis of mRNA expression shows a single abundant mRNA of about 0.8 kb ubiquitously expressed, Western blot analysis of the overexpressed protein shows a band of the expected size equally distributed between cytosol and membranes. (C) 1999 Academic Press.