Electrical characteristics of suburothelial cells isolated from the human bladder
Electrical characteristics of suburothelial cells isolated from the human bladder
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DOI：
10.1097/01.ju.0000108120.28291.eb
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发表时间：
2004-02-01
影响因子：
6.6
通讯作者：
Fry, CH
中科院分区：
文献类型：
--
作者：
Sui, GP;Wu, C;Fry, CH
目的：我们测量的膜电特性，以及从suburoepithelial layer of the blader.Materials和方法：suburoepithelial细胞分离的细胞的三磷酸腺苷的反应，从活检标本的人膀胱胶原酶破坏。在电流和电压钳下进行电生理学测量，以使用具有基于K+的填充溶液的膜片移液管记录膜电位和离子电流。Fura-2测定细胞内[Ca 2 +] i。结果：细胞呈梭形，两端有突起，与上皮细胞不同。波形蛋白染色的细胞，但上皮细胞和平滑肌细胞没有。细胞具有小的膜电容（27 +/- 16 pF）和90 +/- 48 x 10（9）Ω cm（2）的比膜电阻。平均膜电位为-63 +/-14 mV，但细胞显示自发尖峰或膜电位的随机波动。一个小的净内向电流被一个较大的外向电流叠加。通过去除细胞外Ca ~（2+）使内向电流减弱。电流显示大的自发波动，并大大降低了30 mM四乙基氯化铵。腺苷三磷酸（30至100 μ M）引起的内向电流约50 pA和大的细胞内Ca 2 + transients.Conclusions：这些细胞是电活性的，与以前的观察连接蛋白43标记，表明它们可以作为一个电气网络。在产生内向电流后，这种网络中电压分布的定量模型表明，单个细胞不能充当起搏器，而是一组同时激活的细胞可以产生外周兴奋效应，放大原始反应的幅度。这方面的膀胱感觉的影响进行了讨论。
Purpose: We measured the membrane electrical characteristics as well as the response to adenosine triphosphate of cells isolated from the suburothelial layer of the bladder.Materials and Methods: Suburothelial cells were isolated from biopsy samples of human bladder by collagenase disruption. Electrophysiological measurements were done under current and voltage clamp to record membrane potential and ionic currents using patch pipettes with a K+ based filling solution. Intracellular [Ca2+] was measured with Fura-2.Results: Cells were different from epithelial cells by their spindle-shaped appearance with projections at either end. The cells stained for vimentin but epithelial and smooth muscle cells did not. The cells had small membrane capacitance (27 +/- 16 pF) and a specific membrane resistance of 90 +/- 48 x 10(9) Omega cm(2). Average membrane potential was -63 +/- 14 mV but cells showed spontaneous spikes or random fluctuations of membrane potential. A small net inward current was superimposed by a larger outward current. Inward current was attenuated by the removal of extracellular Ca. Outward current showed large spontaneous fluctuations and was greatly decreased by 30 mM tetraethyl ammonium chloride. Adenosine triphosphate (30 to 100 muM) elicited an inward current of about 50 pA and large intracellular Ca2+ transients.Conclusions: These cells are electrically active which, in conjunction with the previous observation of connexin 43 labeling, suggests that they could act as an electrical network. A quantitative model of voltage distribution in such a network after the generation of inward current suggests that individual cells could not act as pacemakers, but rather a group of simultaneously activated cells could exert a peripheral excitatory effect that would amplify the magnitude of the original response. The implications of this in terms of bladder sensation are discussed.