A Gustatory Receptor GR8 Tunes Specifically to D-Fructose in the Common Cutworm Spodoptera litura
A Gustatory Receptor GR8 Tunes Specifically to D-Fructose in the Common Cutworm Spodoptera litura
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斜纹夜蛾中味觉受体 GR8 专门调节 D-果糖
DOI：
10.3390/insects10090272
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发表时间：
2019-09-01
期刊：
影响因子：
3
通讯作者：
Dong, Shuang-Lin
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Long;Yan, Qi;Dong, Shuang-Lin
味觉受体（GRs）在昆虫非挥发性化合物的外周编码中起着至关重要的作用，在昆虫的取食、交配和产卵等多种行为中起着重要作用。然而，对鳞翅目害虫的gr研究很少。本文以斜纹夜蛾（Spodoptera litura）为研究对象，克隆了果糖GR候选基因SlitGR8，并利用实时荧光定量PCR （quantitative real-time PCR, qPCR）测定了该基因的时空表达谱。结果表明，SlitGR8在雄性和雌性成虫的触角以及1、5、6龄幼虫中均有高表达。利用爪蟾卵母细胞系统进一步进行功能分析。在12种测试的糖化合物中，SlitGR8对d -果糖有特异性反应。此外，行为分析表明，雌性和雄性飞蛾对施加在天线上的d -果糖都有伸长喙的反应，但雌性的敏感性高于雄性。该研究结果为进一步阐明斜纹夜蛾味觉的分子机制提供了重要的基础，并为开发进食干扰技术提供了潜在的靶点。
Gustatory receptors (GRs) are crucial in the peripheral coding of the non-volatile compounds in insects, and thus play important roles in multiple behaviors including feeding, mating, and oviposition. However, little research has been done on GRs in lepidopteran pests. In the current work with Spodoptera litura, an important worldwide crop’s pest, a candidate fructose GR gene (SlitGR8) was cloned in full length, and its spatial and temporal expression profiles were determined by quantitative real-time PCR (qPCR). It revealed that SlitGR8 was highly expressed in antennae of both male and female adults, as well as in larva of first, fifth and sixth instar. Functional analyses were further conducted using the Xenopus oocyte system. SlitGR8 responded specifically to D-fructose among 12 tested sugar compounds. In addition, the behavioral assay demonstrated that both female and male moths could respond with proboscis extension behavior to D-fructose applied onto the antenna, but females showed higher sensitivity than males. The results provide an important base for further elucidation of molecular mechanisms of gustation, and a potential target for development of feeding interfering technique in S. litura.