A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction‐denature gradient gel electrophoresis
A new primer for discrimination of arbuscular mycorrhizal fungi with polymerase chain reaction‐denature gradient gel electrophoresis
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DOI：
10.1111/j.1744-697x.2005.00023.x
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发表时间：
2005-06
影响因子：
1.3
通讯作者：
Kouichi Sato;Y. Suyama;M. Saito;K. Sugawara
中科院分区：
文献类型：
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作者：
Kouichi Sato;Y. Suyama;M. Saito;K. Sugawara
本文报道了丛枝菌根真菌（AM）在陆生植物磷循环中的重要作用以及植物群落结构的决定因素。然而，这是几乎不可能的，以确定AM真菌物种定殖根，因为罕见的物种特异性形态。近年来，聚合酶链反应-变性梯度凝胶电泳技术已被应用于AM真菌的种类鉴定。从国立畜牧研究所获得了草原中存在的五种AM真菌的孢子样本。每个种的10个孢子样品的变性梯度凝胶电泳图谱是可重复的。
This article reports that arbuscular mycorrhizal (AM) fungi have significant roles in phosphorus cycles with terrestrial plants as well as determinants of plant community structure. However, it is almost impossible to identify the AM fungal species colonizing roots because of rare species-specific morphology. Recently polymerase chain reaction-denature gradient gel electrophoresis analysis has been applied to identify and characterize the species of AM fungi. Spore samples of five AM fungal species which exist in grassland were obtained from the National Institute of Livestock. Denature gradient gel electrophoresis profiles of 10 spore samples from each species were reproducible.