Molecular identification key for pest species of Scirtothrips (Thysanoptera: Thripidae)
Molecular identification key for pest species of Scirtothrips (Thysanoptera: Thripidae)
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DOI：
10.1603/0022-0493-99.5.1813
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发表时间：
2006-10-01
影响因子：
2.2
通讯作者：
Stouthamer, Richard
中科院分区：
文献类型：
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作者：
Rugman-Jones, Paul F.;Hoddle, Mark S.;Stouthamer, Richard
有效的植物检疫和生物控制措施需要快速准确地识别可能造成巨大经济损失或环境破坏的外来和潜在入侵类群。硬蓟马属包括几种严重的农业害虫，并且由于它们的微小尺寸和隐蔽行为，易于通过植物材料的国际贸易进行未被发现的运输。虽然使用传统的分类方法将标本分配到Scirtothrips属是简单的，但物种的鉴定要困难得多，需要该属的专业知识。此外，一些Scirtothrips物种的有效性是值得怀疑的。因此，需要一种简便、准确、可靠的鉴定方法。在这里，我们提供了一个简单的分子钥匙的基础上的内部转录间隔区1和2（ITS1和ITS2）的核核糖体DNA。单个样本可以通过用通用引物扩增其ITS1和ITS2区域并通过使用标准琼脂糖凝胶电泳测定产物的大小来鉴定，在某些情况下随后用限制性内切酶SacII或PspOM I进行DNA消化。该识别系统的优点是非专业人员能够快速、廉价地识别单个标本。本工作分析的材料收集于美国（加州）、印度、南非、肯尼亚、墨西哥、危地马拉、洪都拉斯、尼加拉瓜、哥斯达黎加、巴拿马、澳大利亚、新西兰和Raiatea（法属波利尼西亚社会群岛）。我们已经确定了七种害虫与分子为基础的方法在这里描述。随着ITS1和ITS2序列的获得，该系统有望扩展到该属的其他成员。我们还提供了两个新的Scirtothrips物种，一个物种来自洪都拉斯和一个物种来自新西兰的分子确认。
Effective plant quarantine and biological control initiatives require rapid and accurate identification of exotic and potentially invasive taxa that may cause high economic losses or environmental damage. The genus Scirtothrips Shull includes several species that are serious agricultural pests, and, because of their minute size and cryptic behavior, prone to undetected transport through international trade of plant material. Although assigning specimens to the genus Scirtothrips is straightforward using traditional taxonomic methods, identification of species is much more difficult and requires expert knowledge of the genus. Furthermore, the validity of some Scirtothrips species is questionable. Therefore, an easy, accurate, and highly reliable technique is desirable for Scirtothrips identification. Here, we provide a simple molecular key based on the internal transcribed spacer regions 1 and 2 (ITS1 and ITS2) of nuclear ribosomal DNA. Individual specimens can be identified by amplification of their ITS1 and ITS2 regions with general primers and determining the size of the products by using standard agarose gel electrophoresis, followed in some instances by DNA digestion with the restriction enzymes SacII or PspOM I. The advantage of this identification system is that nonspecialists are able to quickly and cheaply identify individual specimens. Material analyzed for this work was collected in the United States (California), India, South Africa, Kenya, Mexico, Guatemala, Honduras, Nicaragua, Costa Rica, Panama, Australia, New Zealand and Raiatea (Society Islands French Polynesia). We have identified seven pest species with the molecular-based methods described here. It is hoped that this system can be extended to other members of the genus as their ITS1 and ITS2 sequences become available. We also provide molecular confirmation for two new Scirtothrips species, one species from Honduras and one species from New Zealand.