Analysis of early meiotic events and aneuploidy in nonobstructive azoospermic men: a preliminary report
Analysis of early meiotic events and aneuploidy in nonobstructive azoospermic men: a preliminary report
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DOI：
10.1016/j.fertnstert.2005.08.055
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发表时间：
2006-03-01
影响因子：
6.7
通讯作者：
Chow, V
中科院分区：
文献类型：
--
作者：
Ma, S;Arsovska, S;Chow, V
目的：通过对无精子症患者减数分裂Ⅰ期精母细胞和减数分裂后精子的观察，研究无精子症患者精子发生的过程。设计：联会复合体（SC）、重组和染色体构成的初步分析背景：学术研究环境。患者：3名非梗阻性无精子症男性和1名生育男性干预（S）：通过免疫荧光处理睾丸组织样本，用针对与SC和重组事件相关的蛋白质的抗体进行分析。荧光原位杂交（FISH）的X，Y，和18号染色体上的精母细胞在前期I和减数分裂后的spermatozoes.Main结果Measures（S）：SC的形成和重组减数分裂I，非整倍体。患者和对照组中至少有一个重组灶的XY二价体的频率在统计学上相似（分别为74.2%和82.6%）。所有观察到的粗线期细胞具有正常的XY结构。尽管如此，精子中的性染色体非整倍体率在患者中统计学上显著高于对照组（1.89% vs.0.83%）。结论（S）：免疫细胞学技术与FISH相结合可以增加严重男性因素不育症病因调查的精确度：男性可以正常配对和重组，但仍然产生非整倍体精子。
Objective: To study the events of spermatogenesis in azoospermic men by examining meiosis I spermatocytes and postmeiotic spermatozoa.Design: A preliminary analysis of synaptonemal complex (SC), recombination, and chromosomal constitution (meiotic and postmeiotic) in testicular tissue.Setting: Academic research environment.Patient(s): Three men with nonobstructive azoospermia and one fertile man (vasectomy reversal: control) who underwent testicular sperm extraction in preparation for intracytoplasmic sperm injection (ICSI).Intervention(S): Testicular tissue specimens were processed by immunofluorescence, analysis with antibodies against proteins associated with SC and recombination events. Fluorescence in situ hybridization (FISH) for chromosomes X, Y, and 18 was done on spermatocytes in prophase I and on postmeiotic spermatozoa.Main Outcome Measure(s): SC formation and recombination in meiosis I, aneuploidy.Result(S): The number of autosomal recombination foci in each patient was not statistically significantly different from control. The frequencies of XY bivalents with at least one recombination focus were statistically similar in the patients and control (74.2% vs. 82.6%, respectively). All observed cells in pachytene had normal XY constitutions. In spite of this, the rate of sex-chromosome aneuploidy in spermatozoa was statistically significantly higher in the patients compared with the control (1.89% vs. 0.83%).Conclusion(S): The combination of immunocytologic technology with FISH can add a level of precision in etiologic investigations of severe male factor infertility: men can have normal pairing and recombination but still yield aneuploid spermatozoa.