Hematologic effects of flt3 ligand in vivo in mice
Hematologic effects of flt3 ligand in vivo in mice
复制标题
DOI：
10.1182/blood.v88.6.2004.bloodjournal8862004
复制
发表时间：
1996-09-15
期刊：
影响因子：
20.3
通讯作者：
Lyman, SD
中科院分区：
文献类型：
--
作者：
Brasel, K;McKenna, HJ;Lyman, SD
我们已经研究了用FLT3配体（FL）体内治疗对鼠造血的影响，包括将祖细胞动员到外周血（PB）中。每天用10 Mu G重组人FL注射一次小鼠15天。在第3、5、8、10、15和22天，对骨髓（BM），脾脏和PB的白细胞和菌落形成单位（CFU）的数量杀死并分析。在经FL处理的小鼠中，脾细胞和PB细胞随时间增加。在脾脏中，B细胞，髓样细胞和成核红细胞细胞的增加。在PB中，淋巴细胞，粒细胞和单核细胞有所增加。 FL治疗3天后观察到BM中CFU的最大数量，CFU粒细胞 - 摩果噬细胞（CFU-GM）和CFU颗粒细胞，红细胞，单核细胞，单核细胞，Megakaryocyte（CFU-GEMMM（CFU-GEMMMM），CFU粒细胞 - 摩托噬细胞（CFU-GM）的增加3.7倍和7.3倍。 ）分别与鼠标血清白蛋白（MSA）处理的对照相比。经过8天的治疗后，脾脏CFU-GM和CFU-GEMM的最大123倍和108倍。在第10天，在PB中看到了最大数量CFU-GM和CFU-GEMM，分别增加了537倍和585倍。与CFU-GM和CFU-GEMM的BM，Spleen和Pb相同的时间范围内，形成爆发的单位 - 骨膜（BFU-E）增加了，尽管大小不那么大。原始的第13天CFU-SPELEN（CFU-S）和表型定义的干细胞也被动员到具有类似动力学的FL处理小鼠的Pb中，与CFU-GM和CFU-GEMM相似。从这些研究中，我们得出的结论是，当作为单一药物给药时，FL是造血祖细胞的有效动员到PB中。 （c）1996年美国血液学学会。
We have investigated the effects of in vivo treatment with flt3 ligand (FL) on murine hematopoiesis, including mobilization of progenitors into the peripheral blood (PB). Mice were injected once daily with 10 mu g recombinant human FL for 15 days. On days 3, 5, 8, 10, 15, and 22, mice were killed and analyzed for the number of leukocytes and colony-forming units (CFU) in bone marrow (BM), spleen, and PB. Splenic and PB cellularity increased with time in FL-treated mice. In the spleen, there was an increase in B cells, myeloid cells, and nucleated erythroid cells; in the PB, there was an increase in lymphocytes, granulocytes, and monocytic cells. The maximal number of CFU in the BM was observed after 3 days of FL treatment, giving 3.7- and 7.3-fold increases in CFU-granulocyte-macrophage (CFU-GM) and CFU-granulocyte, erythrocyte, monocyte, megakaryocyte (CFU-GEMM), respectively, compared with mouse serum albumin (MSA)-treated controls. After 8 days of FL treatment, there was a maximal 123- and 108-fold increase in splenic CFU-GM and CFU-GEMM, respectively. The maximal number CFU-GM and CFU-GEMM were seen in PB on day 10, with 537- and 585-fold increases, respectively. Burst-forming units-erythroid (BFU-E) increased in the same time frame as those of CFU-GM and CFU-GEMM in BM, spleen, and PB, although the magnitude was not as great. Primitive day-13 CFU-spleen (CFU-S) and phenotypically defined stem cells were also mobilized into the PB of FL-treated mice with similar kinetics and magnitude to that of CFU-GM and CFU-GEMM. We conclude from these studies that FL, when administered as a single agent, is a potent mobilizer of hematopoietic progenitors into the PB. (C) 1996 by The American Society of Hematology.