Comparative Network Analysis of Patients with Non-Small Cell Lung Cancer and Smokers for Representing Potential Therapeutic Targets.
Comparative Network Analysis of Patients with Non-Small Cell Lung Cancer and Smokers for Representing Potential Therapeutic Targets.
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DOI：
10.1038/s41598-017-14195-1
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发表时间：
2017-10-23
影响因子：
4.6
通讯作者：
Rahimi Jamnani F
中科院分区：
文献类型：
--
作者：
Pazhouhandeh M;Samiee F;Boniadi T;Khedmat AF;Vahedi E;Mirdamadi M;Sigari N;Siadat SD;Vaziri F;Fateh A;Ajorloo F;Tafsiri E;Ghanei M;Mahboudi F;Rahimi Jamnani F
吸烟是全球肺癌的主要诱因。在这项研究中，我们评估了非小细胞肺癌（NSCLC）患者和吸烟者（SM）的血清自身抗体（AAb）谱，从而确定了参与NSCLC发病机制的过度激活途径和枢纽。对NSCLC组和SM组血清中纯化的免疫球蛋白G进行表面和溶液生物筛选。共检测到20个NSCLC和12个SM特异性肽，用于生成NSCLC和SM蛋白数据集。使用STRING和Gephi可视化NSCLC和sm相关蛋白，并使用enrichment分析其模块。通过将代表性过强的途径，如癌症途径、上皮生长因子受体、c-Met、白细胞介素4 （IL-4）和IL-6信号通路，以及一组蛋白质（如磷脂酶D （PLD）、IL-4受体、IL-17受体、层粘蛋白、胶原蛋白和粘蛋白）整合到PLD途径和炎症细胞因子网络中，作为两组中最关键的事件，我们建立了两个超级网络来阐明NSCLC发病机制的新方面，并确定吸烟对肿瘤形成的影响。综上所述，使用系统生物学方法对AAb谱进行评估可以描述各种疾病中涉及的隐藏事件。
Cigarette smoking is the leading cause of lung cancer worldwide. In this study, we evaluated the serum autoantibody (AAb) repertoires of non-small cell lung cancer (NSCLC) patients and smokers (SM), leading to the identification of overactivated pathways and hubs involved in the pathogenesis of NSCLC. Surface- and solution-phase biopanning were performed on immunoglobulin G purified from the sera of NSCLC and SM groups. In total, 20 NSCLC- and 12 SM-specific peptides were detected, which were used to generate NSCLC and SM protein datasets. NSCLC- and SM-related proteins were visualized using STRING and Gephi, and their modules were analyzed using Enrichr. By integrating the overrepresented pathways such as pathways in cancer, epithelial growth factor receptor, c-Met, interleukin-4 (IL-4) and IL-6 signaling pathways, along with a set of proteins (e.g. phospholipase D (PLD), IL-4 receptor, IL-17 receptor, laminins, collagens, and mucins) into the PLD pathway and inflammatory cytokines network as the most critical events in both groups, two super networks were made to elucidate new aspects of NSCLC pathogenesis and to determine the influence of cigarette smoking on tumour formation. Taken together, assessment of the AAb repertoires using a systems biology approach can delineate the hidden events involved in various disorders.
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DOI：
10.1073/pnas.171320598
发表时间：
2001-08-14
影响因子：
11.1
作者：
Brichory, FM;Misek, DE;Hanash, SM
通讯作者：
Hanash, SM
影响因子：
4.8
作者：
Block, Ethan R.;Tolino, Michael A.;Klarlund, Jes K.
通讯作者：
Klarlund, Jes K.
DOI：
10.1073/pnas.0408197102
发表时间：
2005-01-11
影响因子：
11.1
作者：
Chen, ML;Pittet, MJ;Khazaie, K
通讯作者：
Khazaie, K
影响因子：
4.8
作者：
Arimura, A;van Peer, M;Rothman, PB
通讯作者：
Rothman, PB
影响因子：
10
作者：
Chapman, C. J.;Murray, A.;Robertson, J. F.
通讯作者：
Robertson, J. F.