Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment
Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment
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DOI：
10.1101/gr.8.3.175
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发表时间：
1998-03-01
期刊：
影响因子：
7
通讯作者：
Green, P
中科院分区：
文献类型：
--
作者：
Ewing, B;Hillier, L;Green, P
大量DNA序列信息的可用性已开始彻底改变生物学的实践。结果，当前的大规模测序输出令人印象深刻，但不足以跟上需求不断增长的步伐，尤其是远远远远远远不足以获得3亿座基于人类基因组序列所需的条件2005年的日期。要实现这一目标，改进的自动化将是必不可少的，尤其重要的是，人类参与序列数据处理的参与被大大减少或消除。在这方面的进展将需要提高数据处理软件的准确性和可靠的准确性措施，以减少人类参与误差纠正的需求，并使人类审查更有效。在这里，我们描述了朝着该目标的一步：一个针对自动测序器痕迹的基本呼叫程序，PHRED，具有提高的精度。 PHRED似乎是与ABI软件相比，达到错误率较低的第一个基本电话计划，在检查的数据集中，在读取，机器运行条件或测序化学测序中，所检查的数据集中的误差少40％-50％。
The availability of massive amounts of DNA sequence information has begun to revolutionize the practice of biology. As a result, current large-scale sequencing output, while impressive, is not adequate to keep pace with growing demand and, in particular, is far short of what will be required to obtain the 3-billion-base human genome sequence by the target date of 2005. To reach this goal, improved automation will be essential, and it is particularly important that human involvement in sequence data processing be significantly reduced or eliminated. Progress in this respect will require both improved accuracy of the data processing software and reliable accuracy measures to reduce the need for human involvement in error correction and make human review more efficient. Here, we describe one step toward that goal: a base-calling program For automated sequencer traces, phred, with improved accuracy. phred appears to be the first base-calling program to achieve a lower error rate than the ABI software, averaging 40%-50% fewer errors in the data sets examined independent of position in read, machine running conditions, or sequencing chemistry.