A set of compatible tac promoter expression vectors
A set of compatible tac promoter expression vectors
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DOI：
10.1016/0378-1119(96)00289-2
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发表时间：
1996-10-24
期刊：
影响因子：
3.5
通讯作者：
Linn, T
中科院分区：
文献类型：
--
作者：
Dykxhoorn, DM;StPierre, R;Linn, T
已经开发了一系列表达载体，它们都含有相同的表达盒，该表达盒由 lacI(q) 基因、tac 启动子、多克隆位点 (MCS) 和下游转录终止子组成。该盒已插入四个不同的质粒主链，每个主链都来自单独的不相容性组并携带不同的耐药基因。因此，这些表达质粒的不同组合可以稳定地维持在一起。
A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacI(q) gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.