Deletions near the albino locus on chromosome 7 of the mouse affect the level of tyrosine aminotransferase mRNA.
Deletions near the albino locus on chromosome 7 of the mouse affect the level of tyrosine aminotransferase mRNA.
复制标题
小鼠 7 号染色体上白化基因座附近的缺失会影响酪氨酸转氨酶 mRNA 的水平。
DOI：
10.1073/pnas.82.9.2866
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发表时间：
1985
影响因子：
11.1
通讯作者：
Gluecksohn-Waelsch,S
中科院分区：
文献类型：
--
作者：
Schmid,W;Müller,G;Schütz,G;Gluecksohn-Waelsch,S
小鼠7号染色体上白化病基因座的重叠染色体缺失影响几种肝酶的表达，包括酪氨酸氨基转移酶（达特; L-酪氨酸：2-酮戊二酸氨基转移酶，EC 2.6.1.5）。用克隆达特DNA在缺失纯合子小鼠中检测达特结构基因的完整性及其表达和糖皮质激素和cAMP的诱导作用。Southern杂交结果表明，正常小鼠和突变小鼠的基因结构无明显差异。在纯合子突变体中检测到达特mRNA的量严重减少。残留的mRNA水平不能调节糖皮质激素或cAMP。我们的结论是，小鼠达特的表达和诱导所需的反式作用控制功能可以分配到染色体区域附近的白化病基因座。
Overlapping chromosomal deletions at the albino locus on chromosome 7 of the mouse affect the expression of several liver enzymes, including tyrosine aminotransferase (TAT; L-tyrosine:2-oxoglutarate aminotransferase, EC 2.6.1.5). With cloned TAT DNA the integrity of the TAT structural gene and its expression and inducibility by glucocorticoids and cAMP were examined in deletion homozygous mice. No difference in the structure of the gene between normal and mutant mice was detected by Southern blotting. Severely reduced amounts of TAT mRNA were detected in homozygous mutants. The residual mRNA levels could not be modulated by glucocorticoids or cAMP. We conclude that a trans-acting control function required for expression and inducibility of mouse TAT can be assigned to the chromosomal region near the albino locus.