Conserved Noncoding Sequences Highlight Shared Components of Regulatory Networks in Dicotyledonous Plants
Conserved Noncoding Sequences Highlight Shared Components of Regulatory Networks in Dicotyledonous Plants
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DOI：
10.1105/tpc.112.103010
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发表时间：
2012-10-01
期刊：
影响因子：
11.6
通讯作者：
Ott, Sascha
中科院分区：
文献类型：
--
作者：
Baxter, Laura;Jironkin, Aleksey;Ott, Sascha
DNA中的保守非编码序列（CNSs）是控制基因表达调控元件的可靠指针。使用比较基因组学方法与四个双子叶植物物种（拟南芥，木瓜[番木瓜]，白杨[杨]，和葡萄[葡萄属葡萄]），我们检测到数百个CNSs拟南芥基因上游。不同的定位，长度和富集的转录因子结合位点表明这些CNSs在转录调控中发挥功能性作用。与CNS相关的一组基因中转录因子的富集与以下假设一致：它们一起形成保守的转录网络的一部分，其功能是调节其他转录因子并控制发育。我们确定了一组启动子，其中调控机制可能是模式生物拟南芥和其他双子叶植物之间共享，提供了进一步研究的重点领域。
Conserved noncoding sequences (CNSs) in DNA are reliable pointers to regulatory elements controlling gene expression. Using a comparative genomics approach with four dicotyledonous plant species (Arabidopsis thaliana, papaya [Carica papaya], poplar [Populus trichocarpa], and grape [Vitis vinifera]), we detected hundreds of CNSs upstream of Arabidopsis genes. Distinct positioning, length, and enrichment for transcription factor binding sites suggest these CNSs play a functional role in transcriptional regulation. The enrichment of transcription factors within the set of genes associated with CNS is consistent with the hypothesis that together they form part of a conserved transcriptional network whose function is to regulate other transcription factors and control development. We identified a set of promoters where regulatory mechanisms are likely to be shared between the model organism Arabidopsis and other dicots, providing areas of focus for further research.