Comparative analysis of two ferritin subunits from blunt snout bream (Megalobrama amblycephala): Characterization, expression, iron depriving and bacteriostatic activity
Comparative analysis of two ferritin subunits from blunt snout bream (Megalobrama amblycephala): Characterization, expression, iron depriving and bacteriostatic activity
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团头鲂（Megalobrama amblycephala）两个铁蛋白亚基的比较分析：特征、表达、缺铁和抑菌活性
DOI：
10.1016/j.fsi.2017.05.032
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发表时间：
2017-07-01
影响因子：
4.7
通讯作者：
Liu, Hong
中科院分区：
文献类型：
--
作者：
Ding, Zhujin;Zhao, Xiaoheng;Liu, Hong
铁是几乎所有生物必需的微量元素，而过量的铁是有毒的，因此维持铁的体内平衡至关重要。铁蛋白作为铁储存蛋白，在铁代谢中起着重要作用。在本研究中，我们克隆并鉴定了大头蛇头铁蛋白H亚基，称为MamFerH。在MamFerH的5'未翻译区（UTR）预测了一个铁响应元件（IRE），但其凸起结构与报道的铁蛋白M亚基（MamFerM）不同。MamFerH和MamFerM基因在发育早期表现出相似的表达模式，并在孵化后特异高表达，但其组织表达模式不同。其中，MamFerM在脾脏、肝脏和肾脏中高表达，而MamFerH主要在血液和大脑中表达，说明它们的功能不同。此外，在嗜水气单胞菌感染时，这两个基因的表达在转录和翻译水平上都被诱导，且MamFerH的表达效率更高。免疫组织化学和免疫荧光分析证实，感染后它们在肝脏中的蛋白水平和分布发生了显著变化，表明它们参与了宿主的免疫应答。抑菌实验表明，重组MamFerH对嗜水单胞菌的生长具有更明显的抑制作用。(C) 2017 Elsevier Ltd.版权所有。
Iron is an essential microelement for almost all living organisms, while an excess of iron is toxic, thus maintenance of iron homeostasis is vital. As iron storage protein, ferritin plays an important role in iron metabolism. In the present study, we cloned and characterized the ferritin H subunit from Megalobrama amblycephala, termed as MamFerH. An iron-responsive element (IRE) was predicted in the 5' untranslated region (UTR) of MamFerH, while its bulge structural was different from that of the reported ferritin M subunit (MamFerM). The MamFerH and MamFerM genes exhibited similar expression patterns during early development with specifically high expression post hatching, whereas their tissue expression patterns were different. Specifically, MamFerM was highly expressed in the spleen, liver and kidney, while MamFerH was predominantly expressed in the blood and brain, indicating their different functions. In addition, the expression of the two genes was induced upon Aeromonas hydrophila infection at both transcriptional and translational levels, and MamFerH was more efficient. Immunohistochemistry and immunofluorescence analysis confirmed their significant changes at protein level and distribution in the liver post infection, indicating their participation in host immune response. Furthermore, bacteriostatic experiment revealed that recombinant MamFerH displayed more significant inhibitory effect on the growth of A. hydrophila. (C) 2017 Elsevier Ltd. All rights reserved.