Complete nucleotide sequence and molecular characterization of hemolysin II gene from Bacillus cereus
Complete nucleotide sequence and molecular characterization of hemolysin II gene from Bacillus cereus
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DOI：
10.1016/s0378-1097(99)00455-3
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发表时间：
1999-11-01
影响因子：
2.1
通讯作者：
Solonin, AS
中科院分区：
文献类型：
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作者：
Baida, G;Budarina, ZI;Solonin, AS
测定了蜡状芽孢杆菌VKM-B771溶血素II基因的全序列。推导的初级翻译产物由412个氨基酸残基组成，对应于M-r为45.6 kDa的蛋白质。预测的成熟Hly-II蛋白（残基32至412）是42.3 kDa，这是在密切的协议与微型细胞电泳分析。缺失96个C-末端残基的Hly-II缺失变体仍具有溶血活性。蛋白质一级结构分析显示与任何已知的芽孢杆菌溶细胞素没有同源性。在溶血素II与金黄色葡萄球菌α-毒素、γ-溶血素（HlgB）和杀白细胞素（LukF、LukF-R、LukF-PV）之间发现显著的一般同源性（31-28%同一性）。这些数据表明，溶血素II属于P-通道形成溶细胞素的组。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Hemolysin II gene from Bacillus cereus VKM-B771 has been sequenced. The deduced primary translation product consists of 412 amino acid residues which corresponds to the protein with an M-r of 45.6 kDa. The predicted mature Hly-II protein (residues 32 to 412) is of 42.3 kDa, which is in close agreement with the mini-cell electrophoresis analysis. Hly-II deletion variant lacking 96 C-terminal residues still has hemolytic activity. The protein primary structure analysis revealed no homology with any known Bacillus cytolysins. Significant general homology (31-28% identity) was found between the hemolysin II and Staphylococcus aureus alpha-toxin, gamma-hemolysin (HlgB), and leukocidins (LukF, LukF-R, LukF-PV). The data suggest that hemolysin II belongs to the group of P-channel forming cytolysins. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.