Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on β-Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and β-Amyloid Induced Neurotoxicity in Caenorhabditis elegans.
Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on β-Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and β-Amyloid Induced Neurotoxicity in Caenorhabditis elegans.
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DOI：
10.1007/s11064-016-1998-6
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发表时间：
2016-11
影响因子：
4.4
通讯作者：
Seeram NP
中科院分区：
文献类型：
--
作者：
Ma H;DaSilva NA;Liu W;Nahar PP;Wei Z;Liu Y;Pham PT;Crews R;Vattem DA;Slitt AL;Shaikh ZA;Seeram NP
已发表的数据支持几种含酚天然产品的神经保护作用，包括某些水果，浆果，香料，坚果，绿色茶和橄榄油。然而，有限的数据可用于含酚的植物衍生的天然甜味剂，包括枫糖浆。在此，我们研究了化学标准化的富含酚的枫糖浆提取物（MSX）的神经保护作用，使用生物物理，体外和体内研究的组合。根据生物物理数据（硫磺素T测定、透射电子显微镜、圆二色性、动态光散射和zeta电位），MSX以浓度依赖性方式减少淀粉样β1-42肽（Aβ1-42）纤维化（50-500 μg/mL），具有与神经保护多酚白藜芦醇相似的作用，在其最高试验浓度下（500 μg/mL时为63.5%，50 μg/mL时为77.3%）。MSX（100 μg/mL）可降低小鼠BV-2小胶质细胞中H2 O2诱导的氧化应激（与对照组相比，ROS水平降低16.1%），并下调脂多糖（LPS）刺激的炎症标志物的产生（与对照组相比，NOS、IL-6、PGE 2和TNFα水平分别降低22.1%、19.9%、74.8%和87.6%）。此外，在非接触共培养细胞模型中，将分化的人SH-SY 5 Y神经元细胞暴露于来自用MSX（100 μg/mL）和LPS或单独的LPS处理的BV-2细胞的条件培养基。与从LPS-BV-2处理的细胞收集的培养基相比，MSX-BV-2培养基使SH-SY 5 Y细胞活力增加13.8%。此外，MSX（10 μg/mL）对Aβ1-42诱导的秀丽隐杆线虫神经毒性和麻痹具有保护作用。这些数据支持MSX的潜在神经保护作用，从而促进了对这种天然产品的进一步研究。
Published data supports the neuroprotective effects of several phenolic-containing natural products, including certain fruit, berries, spices, nuts, green tea, and olive oil. However, limited data are available for phenolic-containing plant-derived natural sweeteners including maple syrup. Herein, we investigated the neuroprotective effects of a chemically standardized phenolic-enriched maple syrup extract (MSX) using a combination of biophysical, in vitro, and in vivo studies. Based on biophysical data (Thioflavin T assay, transmission electron microscopy, circular dichroism, dynamic light scattering, and zeta potential), MSX reduced amyloid β1–42 peptide (Aβ1–42) fibrillation in a concentration-dependent manner (50–500 μg/mL) with similar effects as the neuroprotective polyphenol, resveratrol, at its highest test concentration (63.5% at 500 μg/mL vs. 77.3% at 50 μg/mL, respectively). MSX (100 μg/mL) decreased H2O2-induced oxidative stress (16.1% decrease in ROS levels compared to control), and down-regulated the production of lipopolysaccharide (LPS)-stimulated inflammatory markers (22.1, 19.9, 74.8, and 87.6% decrease in NOS, IL-6, PGE2, and TNFα levels, respectively, compared to control) in murine BV-2 microglial cells. Moreover, in a non-contact co-culture cell model, differentiated human SH-SY5Y neuronal cells were exposed to conditioned media from BV-2 cells treated with MSX (100 μg/mL) and LPS or LPS alone. MSX-BV-2 media increased SH-SY5Y cell viability by 13.8% compared to media collected from LPS-BV-2 treated cells. Also, MSX (10 μg/mL) showed protective effects against Aβ1–42 induced neurotoxicity and paralysis in Caenorhabditis elegans in vivo. These data support the potential neuroprotective effects of MSX warranting further studies on this natural product.
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作者：
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通讯作者：
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