Human spermatozoa cryopreservation: comparison of three different freezing protocols.
Human spermatozoa cryopreservation: comparison of three different freezing protocols.
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人类精子冷冻保存：三种不同冷冻方案的比较。
DOI：
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发表时间：
2013
期刊：
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通讯作者：
R. Nappi
中科院分区：
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作者：
C. Omes;Anna Lisa Marchetti;M. L. Masanti;R. Bassani;C. Tinelli;Rossella Chiara Sanarica;A. Spinillo;R. Nappi
人类精子冷冻保存在过去几十年里有了显着改善，但尽管对于保留男性生育能力或治疗严重不育男性很重要，但不同实验室之间的实际方案既不是最佳的也不是标准化的。在本研究中，我们旨在通过比较三种不同的人类精子冷冻保存快速方法，确定在精子活力和活力方面最佳的内部快速冷冻方法。我们的数据表明，与单相或双相冷却相比，M1（三相冷却）是一种与精液质量恶化显着降低相关的方法。统计分析支持了三种协议之间观察到的差异。这些数据强化了先前关于精子质量对体外受精结果影响的证据，并表明改进精子冷冻保存技术的重要性，特别是当精液在基线严重受损时。
Human spermatozoa cryopreservation has significantly improved over the last few decades, but the actual protocols are neither optimal nor standardized between different laboratories in spite of the importance of preserving male fertility or treating severely infertile males. In the present study we aimed to determine the best in-house method of rapid freezing in terms of sperm motility and vitality by comparing three different rapid methods of human spermatozoa cryopreservation. Our data showed that M1 (triphasic cooling) is the method associated with a significantly lower deterioration of semen quality in comparison with mono or biphasic cooling. Differences observed among the three protocols were supported by statistical analysis. These data reinforce previous evidences about the influence of sperm quality on IVF outcome and suggest the importance of improving sperm cryopreservation techniques especially when semen is seriously compromised at baseline.