Continuous-Flow Reactor-Based Enzymatic Synthesis of Phosphorylated Compounds on a Large Scale
Continuous-Flow Reactor-Based Enzymatic Synthesis of Phosphorylated Compounds on a Large Scale
复制标题
DOI：
10.1002/chem.201200101
复制
发表时间：
2012-05-01
影响因子：
4.3
通讯作者：
Wever, Ron
中科院分区：
文献类型：
--
作者：
Babich, Lara;Hartog, Aloysius F.;Wever, Ron
酸性磷酸酶是一种能够催化磷酸基从廉价焦磷酸盐转移到酒精底物的酶，它通过环氧连接体（Immobeads-150或Sepabeads EC-EP）共价固定在聚甲基丙烯酸酯珠上。制动后70？%固定化酶的活力保持不变，固定化酶可稳定保存数月。利用固定化酶，我们能够使用补料分批反应器或连续流动填充床反应器以克规模从相应的伯醇生产和制备D-葡萄糖-6-磷酸、N-乙酰基-D-葡糖胺-6-磷酸、烯丙基磷酸、二羟基丙酮磷酸、甘油-1-磷酸和肌苷-5 '-单磷酸。
Acid phosphatase, an enzyme that is able to catalyze the transfer of a phosphate group from cheap pyrophosphate to alcoholic substrates, was covalently immobilized on polymethacrylate beads with an epoxy linker (Immobeads-150 or Sepabeads EC-EP). After immobilization 70?% of the activity was retained and the immobilized enzyme was stable for many months. With the immobilized enzyme we were able to produce and prepare D-glucose-6-phosphate, N-acetyl-D-glucosamine-6-phosphate, allyl phosphate, dihydroxyacetone phosphate, glycerol-1-phosphate, and inosine-5'-monophosphate from the corresponding primary alcohol on gram scale using either a fed-batch reactor or a continuous-flow packed-bed reactor.