Effects of gonadal hormones and persistent pain on non-spatial working memory in male and female rats
Effects of gonadal hormones and persistent pain on non-spatial working memory in male and female rats
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DOI：
10.1016/s0166-4328(01)00195-4
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发表时间：
2001-08-27
影响因子：
2.7
通讯作者：
Aloisi, AM
中科院分区：
文献类型：
--
作者：
Ceccarelli, I;Scaramuzzino, A;Aloisi, AM
有迹象表明，性腺激素和疼痛对认知功能具有调节作用。我们通过评估性腺切除和持续性疼痛对物体识别测试的影响，在雄性和雌性大鼠中研究了这一问题。完整的去性腺的雄性和雌性大鼠暴露在一个开阔的场地(15分钟)，在其中放置三个物体(试验1)；同样的测试在2小时后重复(试验2)，在用一个新的物体替换一个“熟悉的”物体之后。3天后(第2天)，重复相同的过程(试验3和4，其间间隔2小时)，但有一半的动物在试验3之前立即注射福尔马林。记录每个试验中接近3个对象的潜伏期、频率和持续时间，并通过性别、性腺切除和福尔马林治疗进行比较。结果表明，在所有实验中，切除性腺的雄性和雌性动物对所有物体的接近程度都低于正常动物，运动/探索活动也较少；它们的行为不受重复测试或疼痛的影响。在第一天，完好的雄性比雌性表现出更高的接近新物体的水平。在完整的雄性个体中，第一次和第二次实验之间的2小时延迟没有引起对新物体的探索相对于熟悉的物体的任何显著的修改，而在完整的雌性个体中，对新物体的接近比对熟悉的物体的接近要少得多。同样，在第二天，这个新奇的物体是：完好的雄性比所有其他组接近的时间更长。总之，我们的数据显示，在接受物体识别测试时，循环性腺激素的生理水平对雄性大鼠的表现有显著影响，但对雌性大鼠的影响不大。(C)2001 Elsevier Science B.V.保留所有权利。
There are indications of a modulatory role carried out by gonadal hormones and pain in cognitive functions. We have examined this issue in male and female rats by assessing the impact of gonadectomy and persistent pain on the object recognition test. Intact and gonadectomized male and female rats were exposed to an open field (15 min) in which three objects were placed (Trial 1); the same test was repeated 2 h later (Trial 2), after the replacement of a "familiar" object with a novel one. Three days later (Day 2), the same procedure was repeated (Trial 3 and 4 with 2 h in between) but half of the animals were exposed to formalin-injection immediately before Trial 3. The latency, frequency and duration of approaching the three objects: were recorded in each trial and compared by sex, gonadectomy and formalin treatment. The results showed that gonadectomized males and females had lower levels of approach to all objects and less locomotor/exploratory activity than intact animals in all experimental trials; their behaviour was not affected by repetition of the test or by pain. On Day 1, intact males showed a higher level of approach to the novel object than females. In intact males, the 2 h delay between the first and second trial failed to induce any significant modification of exploration of the novel object with respect to the familiar one, while in intact females the novel object was approached much less than the familiar one. Similarly on Day 2, the novel object was: approached for a longer time by intact males than by all the other groups. In conclusion, our data show that physiological levels of circulating gonadal hormones significantly affected the performance of male but not female rats when exposed to the object recognition test. (C) 2001 Elsevier Science B.V. All rights reserved.