Effects of parasitization by three endoparasitoids on spermatogenesis in their hosts,Plutella xylostella larvae
Effects of parasitization by three endoparasitoids on spermatogenesis in their hosts,Plutella xylostella larvae
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发表时间：
2006
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通讯作者：
Dong-zhang Cai;Su-Fen Bai;Xin Li;Xue-xin Chen
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作者：
Dong-zhang Cai;Su-Fen Bai;Xin Li;Xue-xin Chen
内寄生蜂寄生的寄主昆虫常发生寄生性去势。采用形态学方法和蛋白质技术，研究了小菜蛾（Plutella xylostella）被苏氏潜蝇（Oomyzus sokolowskii Kurdumov）、半闭弯尾姬蜂（Diadegma semiclausum Hell 6 n）和菜蛾绒茧蜂（Cotesia plutellae（Kurdj.）（Hyactera：Braconidae）。结果表明，与对照相比，被O. sokolowskii。然而，当被D.半闭壳小菜蛾幼虫睾丸发育受到影响，生殖细胞核变形，染色质浓缩，但仍能形成细长精子;一对睾丸中蛋白质含量显著下降，但蛋白质组成与对照相比无明显变化。寄生性去势作用在被C. plutellae，被寄生幼虫的精子发生被完全抑制，萎缩的精巢中有大量退化的精巢细胞和精子碎片，精巢中总蛋白含量急剧下降。此外，睾丸总蛋白的SDS-PAGE图谱显示，在小菜蛾幼虫睾丸中缺乏一种分子量约为63.4kD的蛋白。plutellae相比，非寄生的主机幼虫。
Parasitic castration often takes place in host insects parasitized by endoparasitoids. Using the morphological method and protein technology, we studied the effects of parasitization on the testicular development of Plutella xylostella parasitized by Oomyzus sokolowskii Kurdumov （Hymenoptera：Eulophidae）, Diadegma semiclausum Hell6n （Hymenoptera ：Ichneumonidae） or Cotesia plutellae （Kurdj.） （Hymenoptera ：Braconidae）, respectively. The results showed that neither the testicular appearance and structure of the host larvae, nor the content and categories of proteins extracted from its host testes changed compared with the control when the host larvae were parasitized by O. sokolowskii. However, when parasitized by D. semiclausum, the testicular development of P. xylostella larvae was adversely affected with deformed nuclei and condensation of chromatin observed in germ cells, but some elongated sperms could still come into being in the testes of parasitized larvae; the content of proteins in a pair of testes decreased significantly after parasitization, but the constitution of proteins had no alteration compared with that of the control. The most pronounced parasitic castration was observed in the host larvae parasitized by C. plutellae, spermatogenesis in the parasitized larvae was inhibited completely, many degenerated testicular cells and the debris of sperm were found in the atrophic testes, and the content of total proteins in these testes sharply declined. Furthermore, SDS-PAGE profiles of testicular total proteins revealed that a protein of an approximate molecular mass of 63.4 kD was absent in testes of P. xylostella larvae parasitized by C. plutellae compared with the non-parasitized host larvae.