Sex-dependent activity of de novo methyltransferase 3 (Tudnmt3) in the two-spotted spider mite, Tetranychus urticae
Sex-dependent activity of de novo methyltransferase 3 (Tudnmt3) in the two-spotted spider mite, Tetranychus urticae
复制标题
二斑叶螨中 de novo 甲基转移酶 3 (Tudnmt3) 的性别依赖性活性
DOI：
10.1111/imb.12120
复制
发表时间：
2014
影响因子：
2.6
通讯作者：
Xiao-Yue Hong
中科院分区：
文献类型：
--
作者：
Si-Xia Yang;Chao Guo;Min Xu;Jing-Tao Sun;Xiao-Yue Hong
DNA methylation is an epigenetic mechanism for regulating developmental and other important processes in eukaryotes. Several components of the DNA methylation machinery have been identified, such as DNA methyltransferases. However, little is known about DNA methyltransferases in chelicerates, which is the second largest arthropod group. Epigenetics are expected to have a crucial role in the metabolism and development of this group. Here, we investigated the role of DNA methyltransferase 3 in the development ofTetranychus urticaeKoch.In silicoanalyses clearly showed that this enzyme possesses the necessary conserved motifs for the catalytic activity ofde novomethylation of DNA. Real‐time PCR revealed thatT. urticae de novo methyltransferase 3(Tudnmt3) is expressed ubiquitously and throughout the life cycle of the two‐spotted spider mite. However, the pattern ofTudnmt3expression was sex‐dependent during the adult stage. Wholein situhybridization provided supportive evidence thatTudnmt3is linked to the differentiation of the gonads in adult females and males. Methylation‐sensitive amplification polymorphism analyses of 119 loci showed that the status of DNA methylation is partially different between adult females and males, raising the possibility that this sex‐dependent DNA methylation pattern is mediated by different methylation activity ofTudnmt3.
