Ultraviolet-B-induced stress and changes in gene expression in Arabidopsis thaliana:: role of signalling pathways controlled by jasmonic acid, ethylene and reactive oxygen species
Ultraviolet-B-induced stress and changes in gene expression in Arabidopsis thaliana:: role of signalling pathways controlled by jasmonic acid, ethylene and reactive oxygen species
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DOI：
10.1046/j.1365-3040.1999.00499.x
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发表时间：
1999-11-01
影响因子：
7.3
通讯作者：
Thomas, B
中科院分区：
文献类型：
--
作者：
Mackerness, SAH;Surplus, SL;Thomas, B
补充UV-B的处理导致拟南芥光合作用基因Lhcb和psbA的转录减少，同时两个病原体相关基因PR-1和PDF1.2的转录增加。UV-B辐射引起茉莉酸（JA）水平和乙烯产生量的增加。对JA和乙烯不敏感的突变体jar 1和etr 1 -1的UV-B辐射处理表明，JA和乙烯对PR-L转录的增加依赖于乙烯和PDF1.2转录的增加，而JA和乙烯对光合作用转录的下调不依赖于这两种化合物。前期研究表明活性氧（reactive oxygen species，ROS）在W-B诱导的光合作用基因下调和PR-1基因上调中发挥作用。在这里，我们已经表明，活性氧也需要紫外线B诱导的PDF1.2基因的上调。结果表明，UV-B对这三组基因的影响是通过三个不同的信号转导途径介导的，这些途径与病原体感染引起的途径相似，但不相同。此外，增加敏感性的jar 1和etr 1 -1突变体的UV-B辐射，与温和型植物相比，表明完整的JA和乙烯信号通路所需的防御UV-B损伤。
Treatment with supplementary UV-B resulted in decreases in transcripts of the photosynthetic genes Lhcb and psbA and concomitant increase in transcripts of two pathogen-related genes, PR-1 and PDF1.2, in Arabidopsis thaliana. UV-B exposure caused increases in jasmonic acid (JA) levels and ethylene production, UV-B treatment of jar1 and etr1-1 mutants, which are insensitive to JA and ethylene, respectively, showed that the increase in PR-L transcripts was dependent on ethylene and PDF1.2 transcripts on both JA and ethylene, In contrast, the down-regulation of photosynthetic transcripts was independent of both compounds. Previous studies have indicated a role for reactive oxygen species (ROS) in the W-B-induced down-regulation of the photosynthetic genes and up-regulation of PR-1 genes. Here we have shown that ROS are also required for the UV-B-induced up-regulation of PDF1.2 genes. The results indicate that the effects of UV-B on the three sets of genes are mediated through three distinct signal transduction pathways which are similar, but not identical, to pathways initiated in response to pathogen infection. In addition, the increased sensitivity of both jar1 and etr1-1 mutants to UV-B radiation, as compared with mild-type plants, indicated that intact JA and ethylene signal pathways are required for defence against UV-B damage.