Immunohistochemical identification of adrenomedullin in human, rat, and porcine tissue
Immunohistochemical identification of adrenomedullin in human, rat, and porcine tissue
复制标题
人、大鼠和猪组织中肾上腺髓质素的免疫组织化学鉴定
DOI：
--
复制
发表时间：
1995
影响因子：
2.3
通讯作者：
T. Eto
中科院分区：
文献类型：
--
作者：
H. Washimine;Y. Asada;K. Kitamura;Y. Ichiki;S. Hara;Yoshitaka Yamamoto;K. Kangawa;A. Sumiyoshi;T. Eto
研究了肾上腺髓质素 (AM) 的组织学定位，这是一种最初从人嗜铬细胞瘤中分离出来的新型血管舒张肽。使用针对包含人肾上腺髓质素 [AM(40-52)NH2] C 端氨基酸 40-52 的片段的多克隆抗体，检查了 AM 在人、大鼠和猪组织中的免疫组织学分布。几乎所有三个物种的人类嗜铬细胞瘤和正常肾上腺髓质细胞都经过免疫染色，结果发现 AM 呈强阳性。此外，AM免疫反应性细胞存在于胰岛、胃肠神经内分泌系统、垂体前叶和脉络丛中，具有一定程度的种间异质性。这些发现表明AM免疫反应细胞广泛分布于内分泌和神经内分泌系统中，表明AM在控制全身和局部循环以及体液分泌方面发挥着重要作用。
The histological localization was investigated of adrenomedullin (AM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma. The immunohistological distribution was examined of AM in human, rat, and procine tissues using a polyclonal antibody to a fragment comprising C-terminal amino acids 40–52 of human adrenomedullin [AM(40–52)NH2]. Almost all of the human pheochromocytoma and normal adrenal medullary cells of all three species were immunostained and found to be intensely positive for AM. Furthermore, AM-immunoreactive cells were present in the pancreatic islets, gastrointestinal neuroendocrine system, anterior pituitary, and choroid plexus with some degree of interspecies heterogeneity. These findings indicate that AM-immunoreactive cells are widely distributed in the endocrine and neuroendocrine system, suggesting that AM plays some important role in the control of systemic and local circulation and also of humoral secretion.