Inflammatory responses of gingival epithelial cells stimulated with Porphyromonas gingivalis vesicles are inhibited by hop-associated polyphenols.
Inflammatory responses of gingival epithelial cells stimulated with Porphyromonas gingivalis vesicles are inhibited by hop-associated polyphenols.
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DOI：
10.1902/jop.2008.070364
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发表时间：
2008
影响因子：
4.3
通讯作者：
Y. Kou;H. Inaba;Takahiro Kato;M. Tagashira;Daiki Honma;T. Kanda;Y. Ohtake;A. Amano
中科院分区：
文献类型：
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作者：
Y. Kou;H. Inaba;Takahiro Kato;M. Tagashira;Daiki Honma;T. Kanda;Y. Ohtake;A. Amano
背景牙周炎是由细菌毒力和宿主防御能力失衡引起的。牙龈卟啉单胞菌是一种主要的牙周病原菌，可引发一系列宿主炎症反应，加重牙周组织的破坏。因此，抗炎药物被认为是有效牙周治疗的理想药物。在本研究中，我们研究了啤酒花苞片多酚(HBP)对牙龈假单胞菌细胞膜囊泡诱导的细胞炎症反应的抑制作用。方法用牙龈假单胞菌膜泡刺激永生化人牙龈上皮细胞，用实时荧光定量逆转录-聚合酶链式反应检测HBP对人牙龈上皮细胞环氧合酶(COX)-2、白介素6(IL)-8、基质金属蛋白酶(MMP)-1、-3mRNA表达的影响。结果HBP以剂量依赖方式抑制COX-2、IL-6和IL-8以及基质金属蛋白酶-1和3的mRNA表达，而表没食子儿茶素没食子酸酯(对照多酚)仅抑制COX-2mRNA的表达。进一步分离鉴定有效成分后，2-[(2-methylpropanoyl)-phloroglucinol]1-O-beta-D-glucopyranoside(MPPG)被鉴定为完全抑制炎症基因诱导的显著抗炎成分。山奈酚3-O-β-吡喃葡萄糖苷(黄酮苷)也有抗炎作用。结论HBP是一种有效的抑制牙龈假单胞菌囊泡诱导的细胞炎症反应的药物。此外，MPPG和黄芪甲苷在此被确认为HBP的有效成分，也可能对预防和/或减轻牙周炎有用。
BACKGROUND Periodontitis is induced by an imbalance between bacterial virulence and host defense ability. Porphyromonas gingivalis, a predominant periodontal pathogen, triggers a series of host inflammatory responses that aggravate the destruction of periodontium. Thus, anti-inflammatory reagents are considered desirable for effective periodontal therapy. In the present study, we examined the inhibitory effects of hop bract polyphenol (HBP) on cellular inflammatory responses induced by P. gingivalis membrane vesicles. METHODS Immortalized human gingival epithelial cells were stimulated with P. gingivalis membrane vesicles, and the effects of HBP on mRNA expression of cyclooxygenase (COX)-2, interleukin (IL)-6 and -8, and matrix metalloproteinase (MMP)-1 and -3 were examined using real-time reverse transcription-polymerase chain reaction. RESULTS HBP inhibited the mRNA expression of COX-2, IL-6 and -8, and MMP-1 and -3 in a dose-dependent manner, whereas epigallocatechin gallate (a control polyphenol) inhibited COX-2 mRNA expression only. Following further fractionation of HBP to identify the effective components, 2-[(2-methylpropanoyl)-phloroglucinol]1-O-beta-D-glucopyranoside (MPPG) was identified as a significant anti-inflammatory element that completely inhibited the inflammatory mRNA induction. Kaempferol 3-O-beta-glucopyranoside (astragalin) also was found to have anti-inflammatory effects. CONCLUSIONS HBP is suggested to be a potent inhibitor of cellular inflammatory responses induced by P. gingivalis vesicles. Further, MPPG and astragalin, identified here as effective components of HBP, also may be useful for the prevention and/or attenuation of periodontitis.