Polycyclic aromatic hydrocarbon (PAH) degradation coupled to methanogenesis
Polycyclic aromatic hydrocarbon (PAH) degradation coupled to methanogenesis
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DOI：
10.1007/s10529-005-6073-3
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发表时间：
2006-03-01
影响因子：
2.7
通讯作者：
Holoman, TRP
中科院分区：
文献类型：
--
作者：
Chang, W;Um, Y;Holoman, TRP
巴尔的摩港（巴尔的摩，MD）沉积物被用来启动厌氧富集培养与多环芳烃（PAHs）的情况下，补充电子受体。用萘和菲修正的培养物表现出持久的，可转移的多环芳烃降解。溴乙烷磺酸，甲烷生成的选择性抑制剂，抑制200 μ M萘和菲的降解;基于16 S rRNA序列的分子表征证实，产甲烷菌被消除，从而提供证据表明，甲烷生成参与降解途径。
Baltimore Harbor (Baltimore, MD) sediments were utilized to initiate anaerobic enrichment cultures with polycyclic aromatic hydrocarbons (PAHs) in the absence of supplementary electron acceptors. Cultures amended with naphthalene and phenanthrene exhibited sustained, transferable degradation of the PAHs. Bromoethanesulfonic acid, a selective inhibitor of methanogenesis, inhibited the degradation of 200 mu M naphthalene and phenanthrene; molecular characterization based on 16S rRNA sequences confirmed that methanogenic Archaea were eliminated, thus providing evidence that methanogenesis is involved in the degradation pathway.