Analysis of genes expressed in the mantle of oyster Crassostrea gigas
Analysis of genes expressed in the mantle of oyster Crassostrea gigas
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DOI：
10.1046/j.1444-2906.2002.00473.x
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发表时间：
2002-06-01
影响因子：
1.9
通讯作者：
Okoshi, K
中科院分区：
文献类型：
--
作者：
Miyamoto, H;Hamaguchi, M;Okoshi, K
采用抑制性消减杂交技术从长牡蛎外套膜中分离到347个cDNA。通过北方杂交分析，发现这几个cDNA的mRNA序列在外套膜中高表达。共有61个序列显示出与已知序列的密切相似性，它们被分为七组：（i）蛋白质合成;（ii）细胞骨架;（iii）信号转导;（iv）细胞外基质;（v）代谢;（vi）转录因子;和（vii）其他。64号cDNA与麦哲伦蛤肌动蛋白的相似性高于C. gigas，表明C. gigas有两种肌动蛋白基因的同种型。这项工作是在分子水平上阐明地幔功能活动的第一步。
total of 347 cDNA were isolated from the mantle of the oyster Crassostrea gigas by the suppression subtractive hybridization method. By northern blot analysis, we found the mRNA sequences of the several cDNA were highly expressed in the mantle. A total of 61 sequences showed close similarities to known sequences, and they were classified into seven groups: (i) protein synthesis; (ii) cytoskeleton; (iii) signal transduction; (iv) extracellular matrix; (v) metabolism; (vi) transcription factors; and (vii) others. Number 64 cDNA was more similar to the actin of Placopecten magellanicus than C. gigas, indicating that C. gigas has two isotypes of actin genes. This work is the first step to clarify functional activities of the mantle at the molecular level.