Cooperative Effect of Guanidinium Chloride and Urea on Lysozyme Refolding
Cooperative Effect of Guanidinium Chloride and Urea on Lysozyme Refolding
复制标题
氯化胍和尿素对溶菌酶重折叠的协同作用
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
S. Luo
中科院分区：
文献类型：
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作者：
Haiyan Duan;Xiandong Zeng;B. Tang;Xiaotao Liu;Guohua Lan;Wanzhi. Wei;S. Luo
摘要研究了尿素和盐酸胍（GuHCl）对溶菌酶复性的影响。成功地将溶壁微球菌（Micrococcus lysodeikticus）引入到复性系统中。溶菌酶可以从盐酸胍变性，DTT还原状态复性，在最终蛋白浓度高达0.2 mg·mL−1时，产率为96.54%。利用动力学模型研究了复性过程的动力学行为，并计算了动力学常数。在盐酸胍和尿素共存时，尿素浓度增加到一定值后，聚集速率降低。盐酸胍和尿素的协同作用不仅抑制了聚集反应的竞争，而且有效地提高了复性产率。
Abstract The interaction between urea and guanidinium chloride (GuHCl) on lysozyme refolding was investigated in this work. Live micrococcus lysodeikticus was successfully introduced into a refolding system. Lysozyme can be refolded from the GuHCl-denatured, DTT-reduced state in a good yield of 96.54% at final protein concentration as high as 0.2 mg·mL−1. A model could be employed to elucidate refolding kinetics behavior and the kinetics constants were studied. In the coexistence of GuHCl and urea, the aggregation rate decreased by increasing urea concentration to a proper value. The cooperation of GuHCl and urea not only suppressed the competition of the aggregation reaction but also increased the yield of refolding efficiently.
影响因子：
3.5
作者：
Gurbhele-Tupkar, Minakshi C.;Perez, Lissette R.;Lees, Watson J.
通讯作者：
Lees, Watson J.