Human-mouse alignments with BLASTZ
Human-mouse alignments with BLASTZ
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DOI：
10.1101/gr.809403
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发表时间：
2003-01-01
期刊：
影响因子：
7
通讯作者：
Miller, W
中科院分区：
文献类型：
--
作者：
Schwartz, S;Kent, WJ;Miller, W
小鼠基因组分析联盟使用适合各种需要的比对程序，出于各种目的对人类和小鼠基因组序列进行比对。为了研究有关基因组进化的问题，需要一种特别敏感的方法来比对大部分中性进化区域。我们选择了一个名为BLASTZ的程序，这是一个独立的Gapped BLAST算法，专门用于比对两个长基因组序列。随后对BLASTZ进行了修改，以获得足以比对整个哺乳动物基因组的效率并提高其灵敏度。这项工作描述了BLASTZ，它的修改，我们运行它的硬件环境，和一些实证研究，以验证其结果。
The Mouse Genome Analysis Consortium aligned the human and mouse genome sequences for a variety of purposes, using alignment programs that suited the various needs. For investigating issues regarding genome evolution, a particularly sensitive method was needed to permit alignment of a large proportion of the neutrally evolving regions. We selected a program called BLASTZ, an independent implementation of the Gapped BLAST algorithm specifically designed for aligning two long genomic sequences. BLASTZ was subsequently modified, both to attain efficiency adequate for aligning entire mammalian genomes and to increase its sensitivity. This work describes BLASTZ, its modifications, the hardware environment on which we run it, and several empirical studies to validate its results.