Synthesis and phorbol ester-binding studies of the individual cysteine-rich motifs of protein kinase D.
Synthesis and phorbol ester-binding studies of the individual cysteine-rich motifs of protein kinase D.
复制标题
蛋白激酶 D 的单个富含半胱氨酸基序的合成和佛波酯结合研究。
DOI：
10.1016/s0960-894x(99)00413-8
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发表时间：
1999
影响因子：
2.7
通讯作者：
Kikkawa,U
中科院分区：
文献类型：
--
作者：
Irie,K;Nakahara,A;Ohigashi,H;Fukuda,H;Wender,PA;Konishi,H;Kikkawa,U
为了研究蛋白激酶D（PKD）富含半胱氨酸基序的佛波酯结合特性，合成了含有PKD富含半胱氨酸基序的52肽（PKD-C1 A，PKD-C1 B）。[3 H]佛波醇-12，13-二丁酸酯（PDBu）与PKD-C1 A的结合受孵育温度的影响显著，而与PKD-C1 B的结合不受温度的影响。[3 H]PDBu与PKD C1肽结合的Scatchard分析得出PKD-C1 A和PKD-C1 B的解离常数分别为2.5 ± 0.4和2.7 ± 0.8 nM，表明PKD的两个富含半胱氨酸的基序与PKCγ的基序在功能上等同。
To investigate the phorbol ester-binding properties of the individual cysteine-rich motifs of protein kinase D (PKD), the 52-mer peptides containing each cysteine-rich motif of PKD (PKD-C1A, PKD-C1B) have been synthesized. The [3H]phorbol-12,13-dibutyrate (PDBu) binding to PKD-C1A was affected drastically by incubation temperature while that to PKD-C1B was not. Scatchard analysis of [3H]PDBu binding to both PKD C1 peptides gave dissociation constants of 2.5 ± 0.4 and 2.7 ± 0.8 nM for PKD-C1A and PKD-C1B, respectively, indicating that the two cysteine-rich motifs of PKD are functionally equivalent like those of PKCγ.
蝶啶的化学和生物学：Ciba 基金会研讨会，G.E.W. 编辑
DOI：
10.1016/0006-3002(56)90457-7
发表时间：
1956
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
C. Fromageot
通讯作者：
C. Fromageot