Diffusion of aldolase from rat skeletal muscle; an index of membrane permeability.
Diffusion of aldolase from rat skeletal muscle; an index of membrane permeability.
复制标题
醛缩酶从大鼠骨骼肌中的扩散；
DOI：
10.1152/ajplegacy.1957.190.2.201
复制
发表时间：
1957
影响因子：
--
通讯作者：
K. Zierler
中科院分区：
文献类型：
--
作者：
K. Zierler
先前的研究表明，一种名为醛缩酶的蛋白质从培养在各种培养基中的离体大鼠膈肌中流出。由于在该制备中切断了肌纤维，因此存在关于隔膜渗透性的绝对值的一些问题。这些实验已经重复使用一个小的肌肉与两个腱止点，腓骨长肌。除了确认在缺氧、葡萄糖缺乏、通过将肌肉转移到新鲜培养基而产生的代谢改变以及可能的钾引起的膜去极化期间的渗透性增加之外，还表明磷酸盐缓冲液中的渗透性不比碳酸氢盐缓冲液中的渗透性大，并且流出物中的醛缩酶（作为总肌肉醛缩酶的百分比）随着肌肉重量的增加而降低。后者的关系是预测约从圆柱形纤维的表面/体积比，随着体积的增加而减少。
在Ellen Rogus的技术协助下
It was shown previously that a protein, aldolase, flows from excised rat diaphragm incubated in a variety of media. Because muscle fibers are cut in this preparation there was some question about the absolute values of permeability of the diaphragm. These experiments have been repeated using a small muscle with two tendinous insertions, the peroneus longus. In addition to confirming increased permeability during anoxia, glucose lack, altered metabolism produced by transferring muscle to fresh media, and probable membrane depolarization by potassium, it was shown that there is no greater permeability in phosphate buffer than in bicarbonate buffer and that the aldolase in the effluent (as percentage of total muscle aldolase) decreases as the weight of muscle increases. The latter relation is predicted approximately from surface/volume ratio of cylindrical fibers which decreases as volume increases.
with the technical assistance of Ellen Rogus