Mechanisms of bioprosthetic heart valve calcification
Mechanisms of bioprosthetic heart valve calcification
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DOI：
10.1097/01.tp.0000062864.54455.e5
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发表时间：
2003-04-27
期刊：
影响因子：
6.2
通讯作者：
Song, YC
中科院分区：
文献类型：
--
作者：
Brockbank, KGM;Song, YC
背景在每年进行的组织心脏瓣膜手术中，约有20%使用冷冻保存的人类心脏瓣膜。同种异体移植物失败的病理生理学尚未完全了解。作者提出了一种假设，即在一些同种异体心脏瓣膜接受者中观察到的快速恶化是由冷冻保存期间发生的破坏性间质冰损伤引起的，随后导致瓣膜在复张时加速退化。在皮下、幼年大鼠钙化植入模型中，通过比较标准商业心脏瓣膜冷冻保存方法和无冰、玻璃化冷冻保存方法与新鲜对照，对这一假设进行了检验。采用原子吸收光谱法测定外植体中钙离子浓度。统计学显著钙化（P
Background. Cryopreserved human heart valves are used in approximately 20% of the tissue heart valve procedures performed annually. The pathophysiology of allograft failure is not fully understood. The authors proposed the hypothesis that the rapid deterioration observed in some allograft heart valve recipients is caused by disruptive interstitial ice damage that occurs during cryopreservation and subsequently leads to accelerated valve degeneration on implantation.Methods. This hypothesis was tested by comparison of the standard commercial heart valve freezing method of cryopreservation and an ice-free, vitrification method of cryopreservation with fresh controls in a subcutaneous, juvenile rat implant model of calcification. Calcium concentration in explants was determined by atomic absorption spectroscopy.Results. Statistically significant calcification (P