The Crosstalk between Cytokinin and Auxin Signaling Pathways in the Control of Natural Senescence ofArabidopsis thalianaLeaves
The Crosstalk between Cytokinin and Auxin Signaling Pathways in the Control of Natural Senescence ofArabidopsis thalianaLeaves
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DOI：
10.1134/s1021443720060035
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发表时间：
2020-11-01
影响因子：
1.4
通讯作者：
Kusnetsov, V. V.
中科院分区：
文献类型：
--
作者：
Danilova, M. N.;Doroshenko, A. S.;Kusnetsov, V. V.
叶片的自然衰老是受一定遗传程序控制和多种植物激素调控的有规律的生理过程。细胞分裂素（CKs）在叶片衰老控制中起着至关重要的作用，是这一过程的负调节剂。我们先前报道了拟南芥Heynh的营养生长延长和双敲除tahk 2ahk 3突变体的发展。（L.）编码CK受体的基因。根据本研究中所选基因的转录组学分析和实时RT-PCR的数据，ahk 2ahk 3突变体的叶片衰老延迟伴随着增强的SAUR组的表达（4-281倍）（小生长素上调RNA）基因编码生长素反应蛋白和通过上调SSPP（衰老抑制蛋白磷酸酶）基因，其表达水平显著（137倍）超过野生型转录组。因此，很可能在CK信号的显著抑制下，拟南芥叶片衰老通过与SAUR基因家族和与生长素运输和信号相关的特定基因库的表达变化相关的补偿机制来调节。
Natural senescence of leaves is a regular physiological process subjected to a certain genetic program and controlled by various phytohormones. Cytokinins (CKs) play a crucial role in the leaf-senescence control being negative regulators of this process. We previously reported on the prolonged vegetative growth and the development of a double knockoutahk2ahk3mutant ofArabidopsis thalianaHeynh. (L.) for the genes encoding CK receptors. According to the data of the transcriptomic analysis and real-time RT-PCR of selected genes performed in this study, the delay of leaf senescence inahk2ahk3mutants is accompanied by enhanced (4-281-fold) expression of the group ofSAUR(small auxin-up RNA) genes encoding auxin response proteins and by upregulation of theSSPP(senescence-suppressed protein phosphatase) gene, whose expression level significantly (137-fold) exceeded that in a wild-type transcriptome. Thus, it is quite possible that under significant inhibition of the CK signaling leaf senescence inArabidopsisplants regulated via a compensatory mechanism connected with changes in the expression of theSAURgene family and a certain pool of genes related to the auxin transport and signaling.