Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization
Identification of the pathogenic ciliate Pseudocohnilembus persalinus (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization
复制标题
通过荧光原位杂交鉴定致病性纤毛虫 Pseudocohnilembus persalinus（Oligohymenophorea：Scuticociliatia）
DOI：
10.1016/j.ejop.2013.09.004
复制
发表时间：
2014-02-01
影响因子：
2.9
通讯作者：
Xu, Kuidong
中科院分区：
文献类型：
--
作者：
Zhan, Zifeng;Stoeck, Thorsten;Xu, Kuidong
许多盾纤毛虫被认为是水产养殖中的毁灭性病原体。其中，桃叶拟刺壳菌是一种兼性病原菌，常引起海水养殖鱼类的难治性疾病。虽然传统的银染色方法已被成功地用于识别这些纤毛虫，他们的识别受到阻碍，他们的小尺寸和形态相似的密切相关的物种。我们设计了一种替代的方法，鉴定Ppersalinus使用SSU-rDNA靶向的寡核苷酸探针标记的荧光染料，并优化了荧光原位杂交（FISH）协议。通过来自寡核苷酸探针的强荧光信号，该测定导致明确的鉴定。该方法可用于快速、早期检测养殖水体中宿主鱼及其他敏感生物的红褐腐霉感染。它也可以用于研究这种scuticociliate的地理分布。(C)2013 Elsevier GmbH. All rights reserved.
Many scuticociliatid ciliates are regarded as devastating pathogens in aquaculture. Among these, Pseudocohnilembus persalinus is a facultative pathogen that often results in refractory diseases of mariculture fish. Although traditional silver staining methods have been successfully used to identify these ciliates, their identification is hampered by their small size and their morphological similarity to closely related species. We designed an alternative method of identification of P persalinus using an SSU-rDNA targeted oligonucleotide probe labeled with a fluorochrome, and optimized in a fluorescence in situ hybridization (FISH) protocol. The assay results in a clear identification by strong fluorescence signals from the oligonucleotide probe. The method can be used for quick and early detection of P. persalinus infections on host fish, as well as other susceptible organisms in aquiculture water. It may also be used in studies of the geographical distribution of this scuticociliate. (C) 2013 Elsevier GmbH. All rights reserved.