Highly sensitive detection of free testosterone assisted by magnetic nanobeads and gap-enhanced SERS nanotags.
Highly sensitive detection of free testosterone assisted by magnetic nanobeads and gap-enhanced SERS nanotags.
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DOI：
10.1016/j.colsurfb.2022.112460
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
Bing Liu;Shiya Zheng;Hanyu Tang;Qian Liu;Haitao Li;Bingbing Gao;Xiangwei Zhao;Fei Sun
中科院分区：
文献类型：
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作者：
Bing Liu;Shiya Zheng;Hanyu Tang;Qian Liu;Haitao Li;Bingbing Gao;Xiangwei Zhao;Fei Sun
微量游离睾酮(FT)的定量测定对雄激素相关内分泌疾病的诊断具有重要意义。利用磁性纳米棒(MNBs)和间隙增强表面增强拉曼散射(SERS)纳米标记组成的竞争免疫分析机制，发展了一种用于高灵敏FT分析的有趣的检测方法。以MNBs为检测载体，通过简单的磁选即可实现痕量FT的富集化。以银-金核-壳纳米粒子构建的SERS纳米标签作为定量标记，拉曼指示剂位于银核-金壳界面。结果表明，由于GaP增强的表面增强拉曼散射纳米标记的拉曼信号增强和多层膜的高比表面积比，该方法对FT的检测灵敏度达到12.11fgmL-1，线性动态检测范围较宽，R2值为0.979。利用这种灵敏和准确的方法，所开发的强大的策略为通过分析痕量FT进行疾病快速诊断提供了潜在的应用，也可以为其他分析物的分析项目的开发提供指导。
The quantitative determination of trace free testosterone (FT) is of great significance for the diagnosis of androgen-related endocrine diseases. Herein, a fascinating detection protocol was developed for highly sensitive FT analysis through a competitive immunoassay mechanism, which was composed of magnetic nanobeads (MNBs) and gap-enhanced surface enhanced Raman scattering (SERS) nanotags. With the MNBs as detection carriers, trace FT could be enriched by simple magnetic separation. The SERS nanotag constructed with silver-gold core-shell nanoparticle was acted as quantitative label, and Raman indicators were located at the interface between silver core and gold shell. It is demonstrated that the as-proposed protocol achieves high detection sensitivity for FT of 12.11 fg mL-1, and wider linear dynamic detection range (LDR) in the concentration of 100 fg mL-1to 100 ng mL-1with R2value of 0.979, which is due to the enhanced Raman signal of the gap-enhanced SERS nanotag and the high surface-to-volume ratio of the MNB, respectively. Taking advantages of such sensitivity and accuracy approach, the as-developed powerful strategy presents potential applications for rapid disease diagnosis through analyzing trace levels of FT, and can also provide guidance for the exploitation of analysis project of other analytes.