Impact of polymer tether length on multiple ligand-receptor bond formation
Impact of polymer tether length on multiple ligand-receptor bond formation
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DOI：
10.1126/science.293.5529.465
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发表时间：
2001-07-20
期刊：
影响因子：
56.9
通讯作者：
Marques, CM
中科院分区：
文献类型：
--
作者：
Jeppesen, C;Wong, JY;Marques, CM
细胞粘附的促进剂是配体，它们通常附着在与相邻细胞上的表面受体结合的柔性系链上。结合蒙特卡特模拟、扩散反应理论和生物素-链霉亲和素系统的直接实验（表面力测量），我们量化了聚合物链动力学以及系留配体-受体结合的动力学和空间范围。结果表明，强结合的效率不仅仅取决于受体-配体对的分子结构或结合能，也不取决于聚合物系链的平衡构型，而是取决于其“罕见”的延伸构象。
The promoters of cell adhesion are ligands, which are often attached to flexible tethers that bind to surface receptors on adjacent cells. Using a combination of Monte Carte simulations, diffusion reaction theory, and direct experiments (surface force measurements) of the biotin-streptavidin system, we have quantified polymer chain dynamics and the kinetics and spatial range of tethered ligand-receptor binding. The results show that the efficiency of strong binding does not depend solely on the molecular architecture or binding energy of the receptor-ligand pair, nor on the equilibrium configuration of the polymer tether, but rather on its "rare" extended conformations.