Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection
Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection
复制标题
DOI：
10.1007/s00122-003-1338-5
复制
发表时间：
2003-10-01
影响因子：
5.4
通讯作者：
Park, WD
中科院分区：
文献类型：
--
作者：
Conaway-Bormans, CA;Marchetti, MA;Park, WD
稻瘟病由真菌病原体稻瘟病菌引起，是影响世界各地水稻种植地区的严重疾病。目前鉴定抗稻瘟病种质和后代的方法通常利用表型筛选。然而，表型筛选受到环境条件的影响，一种抗性基因的存在有时可以在表型上掩盖其他赋予同一稻瘟病种抗性的基因。 Pi-z 是位于 6 号染色体短臂上的显性基因，可完全抵抗五种稻瘟病。使用公共数据库中发现的序列数据和基于先前克隆的抗性基因的 P 环、核苷酸结合位点和激酶结构域基序的简并引物对，我们开发了与该基因共分离的基于 PCR 的 DNA 标记。这些标记在多种种质中具有多态性，包括应用水稻育种计划的窄杂交特征。它们现在可以作为传统表型筛选的低成本、高通量替代品，用于直接检测稻瘟病抗性基因，允许基因快速渗入易感品种，以及将多个基因掺入单个品系中以获得更持久的稻瘟病抗性。
Rice blast, caused by the fungal pathogen Pyricularia grisea, is a serious disease affecting rice-growing regions around the world. Current methods for identification of blast-resistant germplasm and progeny typically utilize phenotypic screening. However, phenotypic screens are influenced by environmental conditions and the presence of one resistance gene can sometimes phenotypically mask other genes conferring resistance to the same blast race. Pi-z is a dominant gene located on the short arm of chromosome 6 that confers complete resistance to five races of blast. Using sequence data found in public databases and degenerate primer pairs based on the P-loop, nucleotide binding sites and kinase domain motifs of previously cloned resistance genes, we have developed PCR-based DNA markers that cosegregate with the gene. These markers are polymorphic in a wide range of germplasm, including the narrow crosses characteristic of applied rice-breeding programs. They can now be used as a low cost, high-throughput alternative to conventional phenotypic screening for direct detection of blast resistance genes, allowing rapid introgression of genes into susceptible varieties as well as the incorporation of multiple genes into individual lines for more-durable blast resistance.