Purification of DOPA-Containing Foot Proteins from Green Mussel, Perna viridis, and Adhesive Properties of Synthetic Model Copolypeptides
Purification of DOPA-Containing Foot Proteins from Green Mussel, Perna viridis, and Adhesive Properties of Synthetic Model Copolypeptides
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DOI：
10.1080/00218460903291353
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发表时间：
2009-01-01
影响因子：
2.2
通讯作者：
Yamomoto, Hiroyuki
中科院分区：
文献类型：
--
作者：
Ohkawa, Kousaku;Nagai, Tadahiro;Yamomoto, Hiroyuki
绿贻贝（Perna viridis）的丝状粘连组织被称为足骨。酚腺衍生的蛋白质参与足腺的水下粘附。本研究的目的是鉴定和表征绿豆足部酚腺衍生蛋白。绿假单胞菌足部含有至少8种潜在的前体蛋白，命名为绿假单胞菌足部蛋白(Pvfp)-a至-h。在这些前体中，Pvfp-g和Pvfp-h在苯酚腺中含量较高。这些蛋白质被认为是黏附斑块的重要组成部分，它介导线与底物表面的黏附。Pvfp-g和Pvfp-h均富含L- 3,4-二羟基苯基-丙氨酸（DOPA）、酪氨酸（Tyr）和半胱氨酸（Cys），以及甘氨酸（Gly）和赖氨酸（Lys）。根据Pvfp-g和Pvfp-h的氨基酸组成，合成了含有Cys、Tyr、Gly和Lys四种氨基酸的两种共肽作为仿生模型材料。对所得共肽进行酪氨酸酶催化氧化，然后进行拉伸剪切强度测试。结果表明，Pvfp-g和Pvfp-h中的DOPA和Cys共同促进了蛋白质的快速交联，增强了粘附斑块基质的内聚强度。
The thread-like adhesive tissue of the green mussel, Perna viridis, is referred to as the byssus. The phenol gland-derived proteins are involved in the underwater adhesion of the byssus. The objectives of the present study are identification and characterization of the phenol gland-derived proteins in the foot of P. viridis. P. viridis foot contains at least eight kinds of potential precursor proteins, designated as P. viridis foot proteins (Pvfp)-a to -h. Among the precursors, Pvfp-g and Pvfp-h are found at relatively high levels in the phenol gland. These proteins are considered to be essential components in the adhesive plaque, which mediates adhesion of the thread to the substrate surface. Both Pvfp-g and Pvfp-h are enriched in L--3,4-dihydroxyphenyl--alanine (DOPA), tyrosine (Tyr) and cysteine (Cys), as well as glycine (Gly) and lysine (Lys). According to the amino acid compositions in Pvfp-g and Pvfp-h, two copolypeptides containing four amino acids, Cys, Tyr, Gly, and Lys, were synthesized as the biomimetic model materials. The copolypeptides were subjected to the tyrosinase-catalyzed oxidation, followed by tensile shear strength test. The results suggest that DOPA and Cys in Pvfp-g and Pvfp-h cooperatively contribute to rapid protein cross-linking, enhancing the cohesive strength of the matrix of the adhesive plaque.