Influence of DNA synthesis inhibition on the coordinate expression of core human histone genes during S phase.
Influence of DNA synthesis inhibition on the coordinate expression of core human histone genes during S phase.
复制标题
DNA合成抑制对S期核心人类组蛋白基因协调表达的影响。
DOI：
10.1093/nar/11.22.7927
复制
发表时间：
1983
影响因子：
14.9
通讯作者：
Stein,G
中科院分区：
文献类型：
--
作者：
Plumb,M;Stein,J;Stein,G
已检查从 1 mM 羟基脲的 UNA 合成抑制中恢复的 1n S 期 HeLa 细胞的核心 hlstone mRNA 代谢。使用克隆的人 hlstone 基因作为 hlstone mRNA 定量的探针，对 DNA 合成抑制 1 的响应和恢复显示取决于细胞相对于 DNA 复制起始的位置。 3 H-尿苷掺入恢复细胞中的多个组蛋白mRNA中不超过抑制前的水平，并且由于该掺入在S期早期达到最大1n，因此核心组蛋白mRNA的合成显然与基因组的有序复制相关。恢复后中断的 S 期细胞中存在的总 hlstone mRNA 与对照细胞中存在的总 hlstone mRNA 没有显着差异，并且 histone mRNA 水平与 DNA 合成相对速率 1s 之间的时间和功能耦合维持了 1n 扰动的细胞。
Core hlstone mRNA metabolism has been examined 1n S phase HeLa cells recovering from UNA synthesis Inhibition by 1 mM hydroxyurea. Using cloned human hlstone genes as probes for hlstone mRNA quantitation, the response to and recovery from DNA synthesis Inhibition 1s shown to depend on the position of the cell with respect to the initiation of DNA replication. The incorporation of3H-uridine Into multiple hlstone mRNAs in recovering cells does not exceed preinhibition levels, and as this Incorporation 1s maximal 1n early S phase, the synthesis of core hi stone mRNA is apparently related to the ordered replication of the genome. The total hlstone mRNA present in Interrupted S phase cells after recovery is not significantly different from that present in control cells, and a temporal and functional coupling between hi stone mRNA levels and the relative rate of DNA synthesis 1s maintained 1n perturbed cells.