Chlorogenic Acid Entrapped in Hybrid Materials with High PEG Content: A Strategy to Obtain Antioxidant Functionalized Biomaterials?
Chlorogenic Acid Entrapped in Hybrid Materials with High PEG Content: A Strategy to Obtain Antioxidant Functionalized Biomaterials?
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DOI：
10.3390/ma12010148
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发表时间：
2019-01-01
期刊：
影响因子：
3.4
通讯作者：
Pacifico, Severina
中科院分区：
文献类型：
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作者：
Catauro, Michelina;Barrino, Federico;Pacifico, Severina
生物材料植入后形成的促氧化物质可能是植入物本身失效的原因，因为氧化应激诱导的损伤。在这项工作中，SiO2/聚乙二醇（PEG）/绿原酸（CGA）杂化物合成的溶胶凝胶法与50重量%的聚合物和不同的CGA（5，10，15和20重量%）进行了研究。浸泡在模拟体液中的杂化材料表面形成羟基磷灰石层，表明杂化材料具有生物活性。在三种不同剂量（0.5、1和2 mg）下评价的它们对DPPH和ABTS（2，2 '-连氮基-双（3-乙基苯并噻唑啉-6-磺酸））的自由基清除能力显示出探针和剂量依赖性行为。此外，CGA的抗氧化性能不受高量聚合物的存在的影响。采用MTT法检测NIH 3 T3、HaCaT和SH-SY 5 Y细胞的体外生物相容性。除SH-SY 5 Y外，直接暴露于粉末和形态的细胞的线粒体氧化还原活性百分比表示的细胞活力不受影响，表明杂合体具有干扰肿瘤细胞并选择性地作用于肿瘤细胞的能力。不同材料对大肠杆菌和粪肠球菌的抗菌性能受不同量的天然抗氧化剂组分的影响。
The formation of pro-oxidant species after implantation of biomaterials could be responsible for the failure of the implant itself, because of oxidative stress-induced damage. In this work, the SiO2/polyethylene glycol (PEG)/chlorogenic acid (CGA) hybrids synthesized by the sol gel method with 50 wt% of the polymer and different amounts of CGA (5, 10, 15 and 20 wt%) were studied. The hybrids soaked in simulated body fluid (SBF) showed the formation of hydroxyapatite layers on their surface, suggesting that the hybrids are bioactive. Their radical scavenging capacity towards DPPH and ABTS (2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), evaluated at three different doses (0.5, 1 and 2 mg), showed probe- and dose-dependent behavior. In addition, the antioxidant properties of CGA were not affected by the presence of high amounts of the polymer. The in vitro biocompatibility in three cell lines (NIH 3T3, HaCaT and SH-SY5Y) was assessed by using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Apart from SH-SY5Y, the cell viability expressed as mitochondrial redox activity percentage of cells directly exposed to powders and morphology was not affected, suggesting that the hybrids have the ability to interfere and act selectively against tumor cells. The antibacterial properties of the different materials against Escherichia coli and Enterococcus faecal& were affected by different amounts of the natural antioxidant component.