Molecular cloning and characterization of a 79-kDa iron-repressible outer-membrane protein of Moraxella bovis
Molecular cloning and characterization of a 79-kDa iron-repressible outer-membrane protein of Moraxella bovis
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DOI：
10.1016/s0378-1097(03)00554-8
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发表时间：
2003-08-29
影响因子：
2.1
通讯作者：
Takai, S
中科院分区：
文献类型：
--
作者：
Kakuda, T;Oishi, D;Takai, S
牛摩拉氏菌表达一种铁抑制的79 kDa外膜蛋白lrpA。DNA和N端氨基酸序列分析表明，IRPA与脑膜炎奈瑟菌FrpB、淋球菌胎盘和卡他莫拉菌CopB密切相关。锰诱变和凝胶漂移实验的结果表明，IRPA的转录受到铁摄取调节剂的负调控。在IRPA基因中插入抗生素耐药盒影响了菌株利用牛转铁蛋白和乳铁蛋白的能力。在不同地区的临床分离株中均可检测到irpA，且所有受试菌株的抗原性都是保守的。因此，lrpA有可能成为候选疫苗。(C)2003年欧洲微生物学会联合会。爱思唯尔出版，版权所有。
Moraxella bovis expresses an iron-repressible 79-kDa outer-membrane protein, lrpA. DNA and N-terminal amino acid sequence analysis indicate that IrpA is closely related to FrpB of Neisseria meningitidis, FetA of Neisseria gonorrhoeae and CopB of Moraxella catarrhalis. The results of manganese mutagenesis and a gel-shift assay suggested that the transcription of irpA is negatively regulated by the ferric uptake regulator. The insertion of an antibiotic resistance cassette into the irpA gene affected the strain's ability to utilize bovine transferrin and lactoferrin. IrpA was detected in geographically diverse clinical isolates, and the antigenicity of lrpA was conserved in all the isolates tested. Therefore, lrpA may have potential as a candidate vaccine. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.