Beneficial Endophytic Bacteria-Serendipita indica Interaction for Crop Enhancement and Resistance to Phytopathogens
Beneficial Endophytic Bacteria-Serendipita indica Interaction for Crop Enhancement and Resistance to Phytopathogens
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DOI：
10.3389/fmicb.2019.02888
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发表时间：
2019-12-19
影响因子：
5.2
通讯作者：
Compant, Stephane
中科院分区：
文献类型：
--
作者：
del Barrio-Duque, Alejandro;Ley, Johanna;Compant, Stephane
印度梨形孢霉（Serendipita indica，Piriformospora）是一种内生真菌共生体，具有促进植物生长和抵抗不同逆境的能力。然而，这种真菌在该领域的应用导致了不一致的结果，可能是由于与其他微生物的拮抗作用。在这里，我们研究了从内生细菌群落中分离的单个细菌对S. indica。进一步分析了细菌与S. indica影响植物生长和对植物病原体尖孢镰刀菌（Fusarium oxysporum）和立枯丝核菌（Rhizoctonia solani）的保护。芽孢杆菌属、肠杆菌属和伯克霍尔德氏菌属的细菌菌株对S. Indica在平板上生长，而Mycolicibacterium，Rhizobium，Paenibacillus菌株和来自不同分类群的几种其他细菌刺激真菌生长。进一步探索细菌与S. indica，选择属于分枝杆菌属的四种最有前途的菌株用于进一步的实验。两种接种方式的S. indica和Mycolicibacterium菌株增强了S. indica，进一步促进番茄植株的生长，并减轻由植物病原菌F.尖孢R.索拉尼然而，S. indica和细菌的效果不如单独接种。通过分析分枝杆菌菌株的基因组，我们发现这些细菌编码几个基因，这些基因被预测参与刺激S。籼稻生长、植物发育和对非生物和生物胁迫的耐受性。特别是检测到大量与维生素和氮代谢相关的基因。考虑到植物上和植物内部的多重相互作用，我们在这项研究中表明，一些细菌菌株可能会诱导有益的影响。indica，对植物-真菌共生关系有显著影响。
Serendipita (=Piriformospora) indica is a fungal endophytic symbiont with the capabilities to enhance plant growth and confer resistance to different stresses. However, the application of this fungus in the field has led to inconsistent results, perhaps due to antagonism with other microbes. Here, we studied the impact of individual bacterial isolates from the endophytic bacterial community on the in vitro growth of S. indica. We further analyzed how combinations of bacteria and S. indica influence plant growth and protection against the phytopathogens Fusarium oxysporum and Rhizoctonia solani. Bacterial strains of the genera Bacillus, Enterobacter and Burkholderia negatively affected S. indica growth on plates, whereas Mycolicibacterium, Rhizobium, Paenibacillus strains and several other bacteria from different taxa stimulated fungal growth. To further explore the potential of bacteria positively interacting with S. indica, four of the most promising strains belonging to the genus Mycolicibacterium were selected for further experiments. Some dual inoculations of S. indica and Mycolicibacterium strains boosted the beneficial effects triggered by S. indica, further enhancing the growth of tomato plants, and alleviating the symptoms caused by the phytopathogens F. oxysporum and R. solani. However, some combinations of S. indica and bacteria were less effective than individual inoculations. By analyzing the genomes of the Mycolicibacterium strains, we revealed that these bacteria encode several genes predicted to be involved in the stimulation of S. indica growth, plant development and tolerance to abiotic and biotic stresses. Particularly, a high number of genes related to vitamin and nitrogen metabolism were detected. Taking into consideration multiple interactions on and inside plants, we showed in this study that some bacterial strains may induce beneficial effects on S. indica and could have an outstanding influence on the plant-fungus symbiosis.