In vivo biocompatibility study of ABA triblock copolymers consisting of poly(L-lactic-co-glycolic acid) A blocks attached to central poly(oxyethylene) B blocks.
In vivo biocompatibility study of ABA triblock copolymers consisting of poly(L-lactic-co-glycolic acid) A blocks attached to central poly(oxyethylene) B blocks.
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由聚（L-乳酸-共-乙醇酸）A 嵌段与中心聚（氧乙烯）B 嵌段组成的 ABA 三嵌段共聚物的体内生物相容性研究。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
T. Kissel
中科院分区：
文献类型：
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作者：
B. Ronneberger;W. J. Kao;James M. Anderson;T. Kissel
研究了三种由乳酸-乙醇酸共聚物 A 嵌段和聚氧乙烯 (PEO) B 嵌段组成的 ABA 嵌段共聚物和一种无规乳酸-乙醇酸共聚物 (PLG) 的薄膜，以研究不同聚合物组成和分子量对大鼠笼状植入系统的组织反应、毒性降解产物的出现和膨胀行为的影响。通过定量监测白细胞浓度、细胞外酸和碱性磷酸酶活性，在 21 天的植入期内跟踪炎症组织反应。测定膜表面上粘附的巨噬细胞和异物巨细胞的大小和密度。 ABA 和 PLG 植入物仅引起最小的炎症反应，其特征是与空笼对照相比，植入期间白细胞浓度较低。 PEO的含量对聚合物膜表面贴壁细胞的密度有影响。 PEO 含量和分子量的增加降低了植入期间的细胞密度。正如扫描电子显微镜所证明的，在植入期间没有观察到所有聚合物的降解。我们的结果表明，ABA 嵌段共聚物和 PLG 共聚物在笼式植入物测试系统中具有同样良好的耐受性。
Films of three ABA-block copolymers composed of lactic-co-glycolic acid A blocks and poly(oxyethylene) (PEO) B blocks and one random lactic-co-glycolic acid copolymer (PLG) were studied to investigate the influence of different polymer compositions and molecular weights on the tissue reaction, appearance of toxic degradation products, and swelling behavior in the cage implant system in rats. The inflammatory tissue reaction was followed over a 21-day implantation period by monitoring the leukocyte concentration, the extracellular acid, and alkaline phosphatase activities in a quantitative manner. Size and density of adherent macrophages and foreign body giant cells on the film surfaces were determined. The ABA and PLG implants caused only a minimal inflammatory reaction, as characterized by a low concentration of leukocytes during the implantation period when compared to empty cage controls. The content of PEO had an influence on the density of the adherent cells on the surface of the polymer film. An increase in PEO content and molecular weight decreased the cellular density during the implantation period. As demonstrated by scanning electron microscopy, no degradation was observed for all polymers during the implantation period. Our results demonstrate that the ABA block copolymers and PLG copolymer, are equally well tolerated in the cage implant test system.