Divergent roles of a pair of homologous jumonji/zinc-finger-class transcription factor proteins in the regulation of Arabidopsis flowering time
Divergent roles of a pair of homologous jumonji/zinc-finger-class transcription factor proteins in the regulation of Arabidopsis flowering time
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DOI：
10.1105/tpc.104.025353
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发表时间：
2004-10-01
期刊：
影响因子：
11.6
通讯作者：
Noh, YS
中科院分区：
文献类型：
--
作者：
Noh, B;Lee, SH;Noh, YS
拟南芥的开花受多条途径控制，包括光周期途径和开花基因C(FLC)依赖的途径。在这里，我们报道了一对相关的juonji类转录因子，早花6(ELF6)和早花亲缘6(REF6)，在拟南芥开花调控中发挥着不同的作用。ELF6在光周期途径中起抑制作用，而与ELF6相似性最高的REF6是FLC抑制因子。异位表达研究和表达模式分析表明，ELF6和REF6具有不同的细胞功能，尽管它们的序列相似，但也受到不同的调控。REF6对FLC表达的抑制伴随着FLC染色质中组蛋白的修饰，表明这类蛋白的转录调节活性包括染色质重塑。这份报告展示了这类蛋白质在高等真核生物中的体内功能。
Flowering in Arabidopsis thaliana is controlled by multiple pathways, including the photoperiod pathway and the FLOWERING LOCUS C (FLC)-dependent pathway. Here, we report that a pair of related jumonji-class transcription factors, EARLY FLOWERING 6 (ELF6) and RELATIVE OF EARLY FLOWERING 6 (REF6), play divergent roles in the regulation of Arabidopsis flowering. ELF6 acts as a repressor in the photoperiod pathway, whereas REF6, which has the highest similarity to ELF6, is an FLC repressor. Ectopic expression studies and expression pattern analyses show that ELF6 and REF6 have different cellular roles and are also regulated differentially despite their sequence similarities. Repression of FLC expression by REF6 accompanies histone modifications in FLC chromatin, indicating that the transcriptional regulatory activity of this class of proteins includes chromatin remodeling. This report demonstrates the in vivo functions of this class of proteins in higher eukaryotes.