Amphiphilic self-assemblies decorated by nucleobases
Amphiphilic self-assemblies decorated by nucleobases
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DOI：
10.1021/jp0744073
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发表时间：
2007-10-11
影响因子：
3.3
通讯作者：
Baglioni, Piero
中科院分区：
文献类型：
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作者：
Berti, Debora;Bombelli, Francesca Baldelli;Baglioni, Piero
磷脂酰核苷是一类功能性两亲物，其中核酸单体与脂质骨架缀合。这些衍生物在水溶液中自组织成各种尺寸、形状和界面曲率的组装体。本文比较了不同的1-R，2 R-sn-甘油基-3-磷脂酰核苷的聚集行为，其中R = 8（DiC（8）PN）或R = 12（DLPN），N为腺苷（嘌呤）或尿苷（嘧啶），它们是RNA中的互补对。表面张力，小角中子散射，低温透射电镜，和圆二色性被用来突出和区分的疏水组装和碱取代的溶液相行为的影响。我们的主要结论是，核功能化提供了一个额外的参数来控制自组装通过特定的极性头之间的相互作用。进一步的非理想效应是通过将核脂质与互补碱基取代混合来诱导的。我们发现，这些贡献改变聚集阈值和调制属性的聚集在中尺度上。
Phosphatidyl-nucleosides are a class of functional amphiphiles, where a nucleic acid monomer is conjugated to a lipid skeleton. These derivatives self-organize in aqueous solution as assemblies of various size, shape, and interfacial curvature. This paper presents a comparison of the aggregation behavior of different 1-R,2R-sn-glycero-3-phosphatidyl-nucleosides, where R = 8 (DiC(8)PN) or R = 12 (DLPN) and N is either adenosine (a purine) or uridine (a pyrimidine), a complementary pair in RNA. Surface tension, small angle neutron scattering, cryo-TEM, and circular dichroism are used to highlight and distinguish the impact of the hydrophobic assembler and of the base substitution on the solution phase behavior. Our main conclusion is that the nucleic functionalization provides an additional parameter to control self-assembly through specific interactions among the polar heads. Further nonideal effects are induced by mixing nucleolipids with complementary base substitution. We show that these contributions alter the aggregation thresholds and modulate properties of the aggregates on the mesoscale.