Immunohistochemical localization of cholecystokinin in the medial preoptic area and anterior hypothalamus of the Brazilian gray short-tailed opossum: a sex difference.
Immunohistochemical localization of cholecystokinin in the medial preoptic area and anterior hypothalamus of the Brazilian gray short-tailed opossum: a sex difference.
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巴西灰短尾负鼠内侧视前区和下丘脑前部胆囊收缩素的免疫组织化学定位：性别差异。
DOI：
10.1002/neu.480210505
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Jacobson,CD
中科院分区：
文献类型：
--
作者：
Fox,CA;Adam,DE;WatsonJr,R;Hoffman,GE;Jacobson,CD
我们研究了胆囊收缩素样免疫反应（CCK IR）在巴西灰短尾负鼠（Monodelphis astritica）内侧视前区（MPA）和下丘脑前部（AH）胞体和纤维中的解剖定位。在抗生物素蛋白-生物素、镍增强免疫组织化学技术的帮助下，在MPA和AH内发现CCK IR神经元元件。秋水仙素处理的负鼠的MPA中存在大量CCK IR细胞体。MPA还含有CCK IR纤维丛。定量图像分析显示，非秋水仙素处理的雄性负鼠的脑室周围视前区的光阻测量百分比显著高于非秋水仙素处理的雌性负鼠，表明雄性负鼠中CCK IR神经元元素的密度更高。在下丘脑室周围核（Pe）和视交叉上核（SCh）内也可见CCK阳性神经纤维和胞体。这些结果表明，CCK IR神经元元素内发现的MPA和AH的巴西短尾负鼠。此外，有一个性别二态分布的CCK IR元素内MPA的这种小有袋动物。
We have studied the anatomical localization of cholecystokinin‐like immunoreactivity (CCK IR) in somata and fibers in the medial preoptic area (MPA) and anterior hypothalamus (AH) of the Brazilian gray shorttailed opossum, (Monodelphis domestica). With the aid of an avidin‐biotin, nickel‐enhanced, immunohistochemical technique, CCK IR neuronal elements were found within the MPA and AH. A large number of CCK IR cell bodies were located in the MPA of colchicine‐treated opossums. The MPA also contained a CCK IR fiber plexus. Quantitative image analysis revealed that the periventricular preoptic area of noncolchicine‐treated male opossums had a significantly higher percent of blocked light measurements than that of the noncolchicine‐treated females, indicating a higher density of CCK IR neuronal elements in the males. Neuronal fibers and somata containing CCK IR were also found within the periventricular hypothalamic nucleus (Pe), and the suprachiasmatic nucleus (SCh). These results show that CCK IR neuronal elements are found within the MPA and AH of the Brazilian short‐tailed opossum. Furthermore, there is a sexually dimorphic distribution of CCK IR elements within the MPA of this small marsupial.