Behavior of Dictyostelium amoebae is regulated primarily by the temporal dynamic of the natural cAMP wave.
Behavior of Dictyostelium amoebae is regulated primarily by the temporal dynamic of the natural cAMP wave.
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变形虫盘基网柄菌的行为主要受天然 cAMP 波的时间动态调节。
DOI：
10.1002/cm.970230207
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发表时间：
1992
影响因子：
--
通讯作者：
Soll,DR
中科院分区：
文献类型：
--
作者：
Wessels,D;Murray,J;Soll,DR
盘状变形虫盘状ostelium disideumamoebae对cAMP自然波和模拟时间波响应的瞬时速度图高度相似。这种相似性已被用于推导自然波穿过变形虫的动力学，并且利用计算机辅助的动态图像分析系统表征了变形虫在自然波的不同阶段的行为。在自然波锋面的第一个~ 150秒内，细胞持续向聚集中心移动，瞬时速度高，侧向伪足形成的频率降低。在波浪前的最后30秒和波浪后的前30秒，细胞形状“冻结”，细胞运动、伪足形成和细胞内颗粒运动急剧下降。在波后的最后180秒，伪足形成出现反弹，但其方向是随机的，不会导致净细胞移位。数据表明，所有细胞的行为，但取向在易位阶段是由波的时间动力学介导。数据还表明，朝向聚集中心的定向发生在波的前方，一旦定向，细胞在转运阶段以一种盲目的方式移动。©1992 Wiley‐Liss, Inc。
The instantaneous velocity plots ofDictyostelium discoideumamoebae responding to natural waves and simulated temporal waves of cAMP with periods of 7 min are highly similar. This similarity has been used to deduce the dynamics of a natural wave crossing an amoeba, and the behavior of amoebae has been characterized during the different phases of a natural wave with a computer‐assisted dynamic image analyzing system. During the first ∼150 sec of the front of a natural wave, cells move persistently toward the aggregation center, with high instantaneous velocity and a decreased frequency of lateral pseudopod formation. During the last 30 sec of the front of the wave and the first 30 sec of the back of the wave, there is a “freeze” in cell shape and a dramatic depression in cell motility, pseudopod formation, and intracellular particle movement. During the last 180 sec of the back of the wave, there is a rebound in pseudopod formation, but it is random in direction and leads to no net cellular translocation. The data suggest that all of the behavior of a cell but orientation during the translocation phase is mediated by the temporal dynamics of the wave. The data also suggest that orientation toward the aggregation center occurs early in the front of the wave and that, once oriented, cells move in a blind fashion during the translocation phase. © 1992 Wiley‐Liss, Inc.