Impairment of tetanus-specific cellular and humoral responses following tetanus vaccination in human lymphatic filariasis
Impairment of tetanus-specific cellular and humoral responses following tetanus vaccination in human lymphatic filariasis
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DOI：
10.1128/iai.72.5.2598-2604.2004
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发表时间：
2004-05-01
影响因子：
3.1
通讯作者：
Nutman, TB
中科院分区：
文献类型：
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作者：
Nookala, S;Srinivasan, S;Nutman, TB
为探讨淋巴丝虫病患者寄生虫特异性免疫应答受损的后果，观察了20例无症状微丝蚴血症（MF）患者、20例慢性淋巴管阻塞/象皮病患者同时感染班氏吴策线虫（Wuchereria bancrofti）对破伤风类毒素（TT）免疫应答的影响（慢性病理学[CP]）和10个地方性正常（EN）对照个体。接种前，EN对照组和CP组的外周血单核细胞（PBMC）对17的增殖反应无显著差异，但MF组对TT的基线增殖反应显著低于EN组或CP组。接种后6个月，EN和CP组对TT的增殖反应变化显著大于MF组。EN组的γ-干扰素（IFN-γ）反应中重申了这种增殖反应的差异，与丝虫感染组相比，EN组在6个月时IFN-γ的产生增加了400%。与IFN-γ应答相反，与EN组的PBMC相比，MF组的PBMC产生显著增加的TT特异性IL-10水平。虽然EN组和MF组之间在基线时TT特异性免疫球蛋白G（IgG）的产生显著增加，但接种后IgG（和IgG 1同种型）应答在各组之间没有差异，而EN组与丝虫感染组相比，TT特异性IgG 2、IgG 3和IgG 4均增加。这些研究表明，同时感染W。班克罗夫特可以通过可能涉及IL-10的机制减弱对无关抗原的免疫应答。
To investigate the consequences of the impaired parasite-specific immune response in lymphatic filariasis, the effect of concurrent Wuchereria bancrofti infection on the immune response to tetanus toxoid (TT) following tetanus vaccination was studied in 20 asymptomatic microfilaremic (MF) patients, 20 patients with chronic lymphatic obstruction/elephantiasis (chronic pathology [CP]), and 10 endemic normal (EN) control individuals at baseline and at 3 and 6 months after TT vaccination. Peripheral blood mononuclear cell (PBMC) proliferative responses to 17 before vaccination were not significantly different between the EN control and CP groups, but the MF group showed significantly lower baseline proliferative responses to TT compared with either the EN or CP group. Six months following vaccination, the change in proliferative response to TT was significantly greater in the EN and CP groups than in the MF group. This difference in proliferative response was reiterated in the gamma interferon (IFN-gamma) response in the EN group, in that they increased IFN-gamma production by 400% at 6 months, in contrast to that seen in the filaria-infected groups. In contrast to the IFN-gamma responses, PBMCs from the MF group produced significantly increased levels of TT-specific IL-10 compared with PBMCs from the EN group. Although there was significantly greater TT-specific immunoglobulin G (IgG) production at baseline between the EN and MF groups, postvaccination IgG (and IgG1 isotype) responses did not differ among the groups, whereas TT-specific IgG2, IgG3, and IgG4 were all increased in the EN group compared with the filaria-infected groups. These studies indicate that concurrent infection with W. bancrofti can diminish the immune response to an unrelated antigen by a mechanism that is likely to involve IL-10.