FLUORESCENT LIPOPROTEIN PROBE
FLUORESCENT LIPOPROTEIN PROBE
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DOI：
10.1016/0003-2697(82)90675-3
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
SCOTT, J
中科院分区：
文献类型：
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作者：
BERGERON, R;SCOTT, J
荧光胆固醇类似物胆固醇-5,7,9（11）三-3- β。-ol用于标记体内（兔）和体外（人）的高密度脂蛋白（HDL）和低密度脂蛋白（LDL）。兔子喂养实验表明，在体内，这些颗粒结合了酯化和非酯化形式的荧光团。使用体外标记技术，可以选择性地结合荧光团的自由形式或自由形式和酯化形式，这取决于在孵育期间血清酯酶的存在或不存在。标记后，使用温度依赖性荧光强度测量来评估LDL和HDL的热致性行为。含有两种形式荧光团的纯化低密度脂蛋白样本（人和兔）观察到在27度之间发生热变性。和32.度。C.然而，在只含有探针非酯化形式的LDL样品中没有观察到这种转变，在任何HDL样品中也没有观察到这种转变，即使含有两种形式的荧光团。这些结果进一步证明，先前报道的LDL的热致转变是由于LDL胆固醇酯核心的重新排序。
The fluorescent cholesterol analog cholesta-5,7,9(11)trien-3-.beta.-ol was used to label high-density (HDL) and low-density lipoprotein (LDL) in vivo (rabbit) and in vitro (human). Rabbit feeding experiments demonstrated that in vivo both the esterified and nonesterified forms of the fluorophore were incorporated by these particles. Using in vitro labeling techniques, it was possible to selectively incorporate either the free form of the fluorophore or both the free and the esterified forms, depending upon the presence or absence of serum esterases during incubation. Subsequent to labeling, the thermotropic behavior of the LDL and HDL was evaluated using temperature-dependent fluorescence intensity measurements. Purified low-density lipoprotein samples (human and rabbit) containing both forms of the fluorophore were observed to undergo a thermotropic transition between 27.degree. and 32.degree. C. However, this transition was not observed in LDL samples containing only the nonesterified form of the probe nor was it observed in any of the HDL samples, even those containing both forms of the fluorophore. These results provide further evidence that the previously reported thermotropic transition in LDL is due to a reordering of the LDL cholesterol ester core.