Ionic liquid-based aqueous biphasic system for lipase extraction
Ionic liquid-based aqueous biphasic system for lipase extraction
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DOI：
10.1039/c0gc00075b
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发表时间：
2011-01-01
期刊：
影响因子：
9.8
通讯作者：
Marrucho, I. M.
中科院分区：
文献类型：
--
作者：
Deive, F. J.;Rodriguez, A.;Marrucho, I. M.
提出了一种基于离子液体(ILS)和高电荷密度无机盐(K2CO3)的双水相体系(ABS)提取脂解酶的方法。以1-烷基-3-甲基咪唑阳离子为基础，结合氯离子、烷基硫酸盐、烷基磺酸盐和醋酸盐，研究了一种典型的绵羊热霉菌脂肪酶(TLL)在一些最常见的亲水性ILS中的活性。考察了几种操作条件对脂肪酶活性和ABS形成的影响。对温度、pH、失活动力学和水分含量等参数进行了评价，提出了一种可行的提取工艺。对酶的失活动力学进行了更深入的分析，并用串联型失活方程对数据进行了模拟。ATR-FTIR研究旨在确定选定ILS中的TLL结构，也提供了对酶失活行为的洞察。
A successful process to extract lipolytic enzymes based on an aqueous biphasic system (ABS), which uses both ionic liquids (ILs) and a high charge-density inorganic salt (K2CO3), is proposed in this work. The activity of a model Thermomyces lanuginosus lipase (TlL) in some of the most common hydrophilic ILs, based on the 1-alkyl-3-methylimidazolium cation, combined with chloride, alkylsulfate, alkylsulfonate and acetate, was investigated. Several operating conditions influencing lipase activity and ABS formation were investigated. Parameters such as temperature, pH, deactivation kinetics and water content were evaluated in order to propose a viable extraction process. A deeper analysis in terms of enzyme deactivation kinetics was carried out, and the data were modelled through a series-type deactivation equation. ATR-FTIR studies aimed at identifying the TlL structure in selected ILs have also provided an insight into the enzyme deactivation behaviour.